孕酮通过NF-κB调控脑损伤后大鼠海马神经元COX-2的表达
Progesterone influence COX-2 expression on hippocampal neurons after traumatic brain injury in rats by NF-κB signaling pathway
孟景山 1司道文 1张作凤 1张宇新 1张子明1
作者信息
- 1. 华北煤炭医学院解剖学教研室,河北,唐山,063000
- 折叠
摘要
目的:研究NF-κB对脑损伤大鼠海马神经元环氧合酶-2(COX-2)的表达调控作用,探讨孕酮(PROG)对脑损伤(TBI)后大鼠海马神经元保护作用及其可能机制.方法:雄性SD大鼠45只随机分为假手术组、损伤组和PROG治疗组,按照改良的Feeney自由落体损伤装置制作大鼠脑损伤模型,用Nissl染色法、免疫组织化学法及免疫蛋白印记法,观察大鼠海马CA1区NF-κB,COX-2以及前列腺素E2(PGE2)的表达变化;并观察孕酮对上述指标的影响.结果:与假手术组大鼠相比,损伤组大鼠海马CA1区NF-κB(24.0±2.5),COX-2(35.9±2.7),PGE2(34.4±2.9)阳性神经元的数量较假手术组NF-κB(1.9±0.9),COX-2(1.5±0.7),PGE2(1.7±0.7)显著增加(P＜0.05);PROG治疗组与损伤组比较,海马CA1区NF-κB(14.2±1.8),COX-2(16.6±2.7),PGE2(16.5±2.0)阳性神经元的数量明显减少(P＜0.05).结论:NF-κB对脑损伤后海马神经元COX-2表达起调控作用;PROG可能通过影响NF-κB而调控COX-2的表达,对大鼠海马神经元起保护作用.
Abstract
AIM:To investigate the effect of progesterone (PROG)on the expression of nuclear faetor-κB(NF-κB)and cyclooxygenase-2(COX-2)in hippocampal neurons after traumatic brain injury(TB1)in rats.METHODS:Forty-five male Spraque-Dawley rats were randomly divided into 3 groups:shamoperated group(n=15),TBI group(n=15)and PROG-treated group(n=15).The rat model of TBI was duplicated with the improved Feeney's method.Nissl staining,immunohistochemistry and Western blot for NF-κB,COX-2 and prostaglandin E2 (PGE2)was used to observe the changes of positive cell number and protein level in hippocampal neurons and their changes after treated with PROG.RESULTS:The number of NF-κB(24.0±2.5),COX-2(35.9±2.7),PGE2(34.4±2.9)immunoreactive neurons significantly increased in hippocampal at 24 h after TBI compared with the number of NF-κB(1.9±0.9),COX-2 (1.5±0.7),PGE2(1.7±0.7)immunoreactive neurons in sham group.In treatment with PROG.the number of NF-κB (14.2±1.8),COX-2(16.6±2.7),PGE2(16.5±2.0)immunoreactive neurons was reduced obviously as compared with the TBI group(P＜0.05).CONCLUSION:NF-κB may play an important role in mediating COX-2 expression in hippocampal neurons after traumatic brain injury in rats,and progesterone may influence the expression of NF-κB and protect the hippocampal neurons.
关键词
脑损伤/孕酮/炎症/核因子NF-κB/环氧合酶-2/前列腺素E2Key words
traumatic brain injury/progesterone/inflammation/NF-κB/COX-2/PGE2引用本文复制引用
基金项目
唐山市科技局资助项目(08130224C)
出版年
2009
NETL
NSTL
维普
万方数据