Progesterone influence COX-2 expression on hippocampal neurons after traumatic brain injury in rats by NF-κB signaling pathway
AIM:To investigate the effect of progesterone (PROG)on the expression of nuclear faetor-κB(NF-κB)and cyclooxygenase-2(COX-2)in hippocampal neurons after traumatic brain injury(TB1)in rats.METHODS:Forty-five male Spraque-Dawley rats were randomly divided into 3 groups:shamoperated group(n=15),TBI group(n=15)and PROG-treated group(n=15).The rat model of TBI was duplicated with the improved Feeney's method.Nissl staining,immunohistochemistry and Western blot for NF-κB,COX-2 and prostaglandin E2 (PGE2)was used to observe the changes of positive cell number and protein level in hippocampal neurons and their changes after treated with PROG.RESULTS:The number of NF-κB(24.0±2.5),COX-2(35.9±2.7),PGE2(34.4±2.9)immunoreactive neurons significantly increased in hippocampal at 24 h after TBI compared with the number of NF-κB(1.9±0.9),COX-2 (1.5±0.7),PGE2(1.7±0.7)immunoreactive neurons in sham group.In treatment with PROG.the number of NF-κB (14.2±1.8),COX-2(16.6±2.7),PGE2(16.5±2.0)immunoreactive neurons was reduced obviously as compared with the TBI group(P＜0.05).CONCLUSION:NF-κB may play an important role in mediating COX-2 expression in hippocampal neurons after traumatic brain injury in rats,and progesterone may influence the expression of NF-κB and protect the hippocampal neurons.
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