Effects of ketamine and ulinastatin on pulmonary surfactants in endotoxininduced acute lung injury rats
AIM:To investigate the protective effects of ketamine and ulinastatin on acute lung injury.METHODS:Sixty Sprague-Dawley rats were randomly divided into 5 groups in average:Ketamine(K)group,Ulinastatin(U)group,K+U group,LPS group and the control group.The ALI models in the front 4 groups were manipulated by twice beats of LPS.Partial pressure of oxygen in artery(PaO_2),and the phosphatidylcholine and surfactant protein A(SP-A)in the bronchoalveolar lavage fluid (BALF)were assayed at 2 and 4 h.Wet/dry weight ratio(W/D)and histopathology were observed,too.RESULTS:Compared with the other groups,the levels of PaO_2,phosphatidylcholine and SP-A in the LPS group were the lowest(P＜0.05),and the ratio of W/D was the highest(P＜0.05).There was no significant statistic difference of the indexes between the K group and the U group(P＞0.05),but better than the LPS group(P＜0.05).All the indexes were the best and stable in the C group and that in the K+U group were just inferior than those(P＜0.05).In all the groups,except of the C group,PaO_2,phosphatidylcholine and SP-A decreased gradually from 2 to 4 hours after the models were set up.PaO_2 was correlated with the concentration of phosphatidylcholine and SP-A in BALF in a high degree(P＜0.01).CONCLUSION:Both of Ketamine and Ulinastatin could inhibit the deduction of SP-A and phosphatidylcholine and improve PaO_2.Applying them in combination may obtain better protective effect in ALI.
NETL
NSTL
万方数据
维普
