氯胺酮和乌司他丁对急性肺损伤大鼠肺泡表面活性物质的影响
Effects of ketamine and ulinastatin on pulmonary surfactants in endotoxininduced acute lung injury rats
曹殿青 1余艳丽 1郭翠容 1刘欣 1夏文芳1
作者信息
- 1. 广东医学院附属医院麻醉科,广东,湛江,524001
- 折叠
摘要
目的:探讨氯胺酮、乌司他丁对急性肺损伤(ALI)的保护作用及其可能机制.方法:60只SD大鼠随机分至5组:氯胺酮组(K组)、乌司他丁组(U组)、氯胺酮+乌司他丁组(K+U组)、模型对照组(LPS组)和空白对照组(C组).采用内毒素(LPS)二次打击法建立ALI大鼠模型后,在2 h和4h两个时间点上,每组随机取6只大鼠,检测PaO_2、肺泡灌洗液(BALF)中磷脂和SP-A浓度,肺组织的湿/干重比值(W/D)以及HE染色.结果:LPS组PaO_2、磷脂及SP-A浓度与其它4组相比最低(P＜0.05),肺组织W/D比值最大(P＜0.05).K组和U组的各指标在各个时间点均无统计学意义(P＞0.05),但是均优于LPS组(P＜0.05).C组的各指标在各个时间点上均最佳(P＜0.05),并均保持平稳.K+U组次之,并与K组、U组相比各指标差异有统计学意义(P＜0.05).各组PaO_2磷脂浓度、SP-A浓度在4 h内均逐渐降低,但差异无统计学意义.PaO_2与BALF中的磷脂浓度、SP-A浓度均呈高度线性相关性(P＜0.01).结论:氯胺酮和乌司他丁均可有效减少ALI过程中肺泡表面活性物质(PS)活性成分SP-A和磷脂的丢失,从而改善PaO_2,对ALI有显著的保护作用.两者联合应用效果更佳.
Abstract
AIM:To investigate the protective effects of ketamine and ulinastatin on acute lung injury.METHODS:Sixty Sprague-Dawley rats were randomly divided into 5 groups in average:Ketamine(K)group,Ulinastatin(U)group,K+U group,LPS group and the control group.The ALI models in the front 4 groups were manipulated by twice beats of LPS.Partial pressure of oxygen in artery(PaO_2),and the phosphatidylcholine and surfactant protein A(SP-A)in the bronchoalveolar lavage fluid (BALF)were assayed at 2 and 4 h.Wet/dry weight ratio(W/D)and histopathology were observed,too.RESULTS:Compared with the other groups,the levels of PaO_2,phosphatidylcholine and SP-A in the LPS group were the lowest(P＜0.05),and the ratio of W/D was the highest(P＜0.05).There was no significant statistic difference of the indexes between the K group and the U group(P＞0.05),but better than the LPS group(P＜0.05).All the indexes were the best and stable in the C group and that in the K+U group were just inferior than those(P＜0.05).In all the groups,except of the C group,PaO_2,phosphatidylcholine and SP-A decreased gradually from 2 to 4 hours after the models were set up.PaO_2 was correlated with the concentration of phosphatidylcholine and SP-A in BALF in a high degree(P＜0.01).CONCLUSION:Both of Ketamine and Ulinastatin could inhibit the deduction of SP-A and phosphatidylcholine and improve PaO_2.Applying them in combination may obtain better protective effect in ALI.
关键词
急性肺损伤/肺泡表面活性物质/氯胺酮/乌司他丁Key words
acute lung injury/pulmonary surfactant/ketamine/ulinastatin引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据