Effects of resveratrol on the cortical neuron suffered from glutamate neurotoxicity
AIM:To investigate the effects of resveratrol on the primary cultured cortical neuron,the mechanism of glutamate neurotoxicity and the influence of resveratrol On the glutamate neurotoxicity.METHODS:We observed the effect of resveratrol at different time points by adding 20 μmol/L resveratrol into the culture medium of primary cultured cortical neurons in vitro.Glutamate was added into the culture medium to induce the neurotoxicity.Resveratrol was added to the culture medium simultaneously with glutamate in order to observe its effects on neurotoxicity.Morphological observation,MTT test,as well as Bax immunocytochemistry staining were performed to evaluate both the glutamate neurotoxicity and the protective effects of resveratrol.RESULTS:20 μmol/L resveratrol had no obvious effects on the livability of cultured cortical neurons in vitro.1 mmol/L glutamate could induce so intense neurotoxicity that many neurons died and the cell livability was greatly decreased.Immunocytochemistry staining indicated that the apoptosis promoting protein Bax was up-regulated by glutamate.20 μmol/L resveratrol could partly inhibit the neurotoxicity of glutamate by preventing neuron from dying;increasing the cell livability and down-regulating the expression of apoptosis promoting protein Bax.CONCLUSION:Ahhough resveratrol(20 μmol/L)had neither positive nor side effects on the primary cultures of cortical neuron,it could inhibit the glutamate neurotoxicity and protect neuron by down-regulating the expression of Bax.
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万方数据
维普
