首页|木黄酮对小鼠胰岛β细胞上电压依赖性钾电流的影响

木黄酮对小鼠胰岛β细胞上电压依赖性钾电流的影响

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目的:研究酪氨酸激酶抑制剂木黄铜活性对胰岛β细胞中电压依赖性钾电流的影响,探讨酪氨酸激酶在胰岛β细胞中的作用.方法:原代培养小鼠胰岛β细胞,经0.1mmol/L酪氨酸激酶抑制剂木黄酮急性处理处理或者慢性处理12 h后,运用全细胞电流记录的方法观察电压依赖性钾电流的改变.结果:木黄酮急性处理可显著抑制小鼠胰岛β细胞的电压依赖性钾电流.木黄酮处理12 h后,小鼠胰岛β细胞的电压依赖性钾电流较对照组明显减小,降低为对照组的59.3%8.5%(P＜0.01,n=6).结论:木黄酮抑制小鼠胰岛β细胞中电压依赖性钾电流,提示酪氨酸激酶活性抑制在胰岛β细胞功能损害中具有重要作用.
Effect of genistein on voltage-gated potassium current in mouse pancreatic islet-cells
AIM:To determine the effect of inactivation of tyrosine kinases on voltage-gated potassium channels in pancreaticcells and to evaluate the function of tyrosine kinases in pancreaticcells.METHODS:Primarily cultured mouse pancreatic isletcells were treated by 0.1 mmol/L genistein acutely or for 12 hours.Voltage-gated potassium currents were recorded with patch clamp techniques in the configuration of perforated whole-cell recording.RESULTS:The whole-cell voltage-gated potassium currents were significantly diminishes by acute treatment with genistein.After treatment by genistein for 12 hours,the wholecell voltage-gated potassium currents were also decreased as compared to the control group.CONCLUSION:Genistein treatment decreases the current of voltage-gated potassium currents in mouse pancreatic-cells,which suggests that inhibition of tyrosine kinases activity plays an important role in the dysfunction of pancreaticcells.

pancreatic-cellsgenisteinVoltage.gated potassium current

毕媛、赵玉峰、刘亚莉、朱运龙、王捷

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广州军区广州总医院医学实验科,广东,广州,510010

第四军医大学基础部生理学教研室,陕西,西安,710033

胰岛β细胞 木黄酮 电压依赖性钾通道

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(21)
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