木黄酮对小鼠胰岛β细胞上电压依赖性钾电流的影响
Effect of genistein on voltage-gated potassium current in mouse pancreatic islet-cells
毕媛 1赵玉峰 2刘亚莉 2朱运龙 2王捷1
作者信息
- 1. 广州军区广州总医院医学实验科,广东,广州,510010
- 2. 第四军医大学基础部生理学教研室,陕西,西安,710033
- 折叠
摘要
目的:研究酪氨酸激酶抑制剂木黄铜活性对胰岛β细胞中电压依赖性钾电流的影响,探讨酪氨酸激酶在胰岛β细胞中的作用.方法:原代培养小鼠胰岛β细胞,经0.1mmol/L酪氨酸激酶抑制剂木黄酮急性处理处理或者慢性处理12 h后,运用全细胞电流记录的方法观察电压依赖性钾电流的改变.结果:木黄酮急性处理可显著抑制小鼠胰岛β细胞的电压依赖性钾电流.木黄酮处理12 h后,小鼠胰岛β细胞的电压依赖性钾电流较对照组明显减小,降低为对照组的59.3%8.5%(P＜0.01,n=6).结论:木黄酮抑制小鼠胰岛β细胞中电压依赖性钾电流,提示酪氨酸激酶活性抑制在胰岛β细胞功能损害中具有重要作用.
Abstract
AIM:To determine the effect of inactivation of tyrosine kinases on voltage-gated potassium channels in pancreaticcells and to evaluate the function of tyrosine kinases in pancreaticcells.METHODS:Primarily cultured mouse pancreatic isletcells were treated by 0.1 mmol/L genistein acutely or for 12 hours.Voltage-gated potassium currents were recorded with patch clamp techniques in the configuration of perforated whole-cell recording.RESULTS:The whole-cell voltage-gated potassium currents were significantly diminishes by acute treatment with genistein.After treatment by genistein for 12 hours,the wholecell voltage-gated potassium currents were also decreased as compared to the control group.CONCLUSION:Genistein treatment decreases the current of voltage-gated potassium currents in mouse pancreatic-cells,which suggests that inhibition of tyrosine kinases activity plays an important role in the dysfunction of pancreaticcells.
关键词
胰岛β细胞/木黄酮/电压依赖性钾通道Key words
pancreatic-cells/genistein/Voltage.gated potassium current引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据