ZZAP和CDP放散DAT阳性红细胞的效果评价
Effect of ZZAP and CDP elution tests on DAT(+)red blood cells
何子毅 1刘赴平 1刘景春 1车嘉林 1师清莲 1刘仁强 1唐斯 1陈晨2
作者信息
- 1. 东莞市中心血站,广东,东莞,523930
- 2. 第四军医大学西京医院输血科,陕西,西安,710033
- 折叠
摘要
目的:比较ZZAP和CDP两种放散方法放散DAT(+)红细胞的效果及放散后红细胞吸收自身抗体的能力,为DAT(+)红细胞的抗体放散方法提供依据.方法:收集本站送检的DAT(+)交叉配血标本,选择抗-IgG型与抗-(IgG+C3d)复合型各20例作为研究对象,分别采用ZZAP和CDP两种放散方法进行抗体放散试验,对放散后红细胞分别进行DAT试验;用放散后的红细胞吸收患者血浆自身抗体后进行交叉配血试验,评价自身抗体的吸收效果.结果:抗-(IgG+C3d)复合型DAT(+)红细胞用ZZAP和CDP放散效果无差别,ZZAP放散抗-IgG型的效果好于抗-(IgG+C3d)复合型(P＜0.05),CDP放散抗-IgG型与抗-(IgG+C3d)复合型的效果无差异;ZZAP放散抗-IgG型和抗-(IgG+C3d)复合型DAT(+)红细胞后的抗体吸收效果均好于CDP(P＜0.05),ZZAP和CDP放散抗-IgG型DAT(+)红细胞后的吸收效果无明显差异,CDP放散抗-IgG型和抗-(IgG+C3d)复合型DAT(+)红细胞后的吸收效果无差别,ZZAP放散抗-IgG型DAT(+)红细胞后的吸收效果均好于抗-(IgG+C3d)复合型(P＜0.05).结论:抗-(IgG+C3d)复合型DAT(+)红细胞用两种方法均不能很好的解离自身抗体,但对抗-IgG型自身抗体的解离用ZZAP好于CDP,提示临床交叉配血试验中对抗-(IgG+C3d)复合型自身抗体最好选择盲配原则的配血方案,对抗-IgG型自身抗体用ZZAP进行吸收放散效果较好.
Abstract
AIM:To compare the effect of ZZAP and CDP elution tests on DAT(+)red blood cells and the absorption effect of the eluted red blood cells.METHODS:Twenty anti-IgG type and twenty anti-(IgG+C3d)type DAT(+)red blood cell samples were obtained from clinic patients and ZZAP and CDP elution tests were conducted respectively.The effects of autoantibody elution on red blood cell memhers by DAT test and the absorption effects of autoantibody absorption in serum with eluted red blood cells by crossmatch tests were compayed.RESULTS:No significant difference was observed in anti-(IgG+C3d)type red blood cells using ZZAP test or CDP elution test.The effect of autoantibody elution in anti-IgG type was significantly better than that in anti-(IgG+C3d)type(P＜0.05)but using CDP elution test,no significant difference was found between anti-(IgG+C3d)type and anti-IgG type red blood cells.The absorption effect of anti(IgG+C3d)type and anti-IgG type red blood cells by ZZAP was better than that by CDP(P＜0.05),but using ZZAP test or CDP test,no significant difference was found in anti-IgG type red blood cells.No significant difference was observed in anti-(IgG+C3d)type and anti-IgG type red blood cells using CDP.The absorption effect of anti-IgG type red blood cells was significantly better than that of anti-(IgG+C3d)type using ZZAP(P＜0.05).CONCLUSION:Anti-(IgG+C3d)type red blood ceils aye not effectively eluted by ZZAP test or CDP test,but ZZAP is better than CDP in eluting anti-IgG type DAT(+)red blood cells.
关键词
木瓜蛋白酶/二硫苏糖醇/磷酸氯喹/放散试验/自身抗体/贫血,溶血性,自身免疫性/coombs试验/红细胞Key words
papain/dithiothreitol/chloroquine phosphate/elution test/autoantibodies/anemia,hemolytic,autoimmune/coombs'test/erythrocyes引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据