Effect of ZZAP and CDP elution tests on DAT(+)red blood cells
AIM:To compare the effect of ZZAP and CDP elution tests on DAT(+)red blood cells and the absorption effect of the eluted red blood cells.METHODS:Twenty anti-IgG type and twenty anti-(IgG+C3d)type DAT(+)red blood cell samples were obtained from clinic patients and ZZAP and CDP elution tests were conducted respectively.The effects of autoantibody elution on red blood cell memhers by DAT test and the absorption effects of autoantibody absorption in serum with eluted red blood cells by crossmatch tests were compayed.RESULTS:No significant difference was observed in anti-(IgG+C3d)type red blood cells using ZZAP test or CDP elution test.The effect of autoantibody elution in anti-IgG type was significantly better than that in anti-(IgG+C3d)type(P＜0.05)but using CDP elution test,no significant difference was found between anti-(IgG+C3d)type and anti-IgG type red blood cells.The absorption effect of anti(IgG+C3d)type and anti-IgG type red blood cells by ZZAP was better than that by CDP(P＜0.05),but using ZZAP test or CDP test,no significant difference was found in anti-IgG type red blood cells.No significant difference was observed in anti-(IgG+C3d)type and anti-IgG type red blood cells using CDP.The absorption effect of anti-IgG type red blood cells was significantly better than that of anti-(IgG+C3d)type using ZZAP(P＜0.05).CONCLUSION:Anti-(IgG+C3d)type red blood ceils aye not effectively eluted by ZZAP test or CDP test,but ZZAP is better than CDP in eluting anti-IgG type DAT(+)red blood cells.
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维普
