中国甘肃地区汉族IL-10基因多态性与胃癌关系
Association of polymorphism of IL-10 and Helicobacter pylori infection to gastric cancer
祝凯华 1李玉民 2刘玲玲 3张丹 2唐小凡 2何文婷 4陈应泰 2赵军2
作者信息
- 1. 沭阳县人民医院普外科,江苏,沭阳,223600
- 2. 兰州大学第二医院,甘肃,兰州,73000
- 3. 兰州大学基础医学院生物化学和分子生物学研究所,甘肃,兰州,73000
- 4. 兰州大学第一医院肿瘤防治中心,甘肃,兰州,73000
- 折叠
摘要
目的:探讨白细胞介素10(IL-10)启动子区-592位点的单核苷酸多态性(SNP)、及幽门螺旋杆菌(Hp)感染与甘肃地区胃癌之间易感性的关系.方法:采用聚合酶链反应-限制性片段长度多态(PCR-RFLP)技术分别对来自甘肃地区人群137例胃癌患者及与其配比的144例对照个体IL-10-592A/C基因多态性进行检测;采用免疫印迹(Immunoblotting)技术对幽门螺旋杆菌进行检测.结果:①对照人群IL-10基因-592位点AA,AC,CC 3种基因型分布频率分别为29.9%,47.9%,22.2%;②胃癌组IL-10 CC基因型携带频率为32.1%显著高于正常对照组22.2%(P＜0.05),携带IL10 CC基因型个体胃癌的发病风险增高到2.19倍;③以IL10 AA基因型并血清幽门螺杆菌免疫印迹阴性的个体为对照,CC基因型并HP感染阳性及CC基因型并Hp CagA阳性患病风险显著增高,分别为对照组的3.75倍,4.51倍.结论:IL-10基因-592位点A/C多态性与胃癌的遗传易感性相关;IL-10A/C基因多态性和Hp感染在胃癌发生发展过程存在加乘交互作用.
Abstract
AIM:To analyze the relationship both-592 site A/C polymorphism in interleukin-10(IL-10)gene and different virulence factors of Helicobacter pylori in a population from Gansu Province and susceptibility to gastric cancer.METHODS:Polymerase chain restriction fragment length polymorphism (PCR-RFLP)were performed to analyze the genotype of the A/Cpolymorphism in its-592 site of promoter region,and Immunoblotting was performed to test H pylori.RESULTS:①The distribution frequency of AA,AC,CC three genotypes were 29.9%,47.9%,22.2%respectively in normal control.②The frequency of CC genotype was conspicuous higher in the gastric cancer (GC)group compared to the control group(P＜0.05),moreover,individuals with the IL-10AC+CC genotype increased 2.19 fold risk for gastric cancer.③Take IL-10 the AA genotype and the H.wlori-immunoblotting negative individual as the comparison,there was an obviously increased risk of developing gastric cancer for those who carried both CC genotype and H.pylori seropositive,CagA positive,increased 3.75,4.51 fold risk respectively.CONCLUSION:Results indicated that there was a relationship between IL-10-592 A/C polymorphism and susceptibility to gastric cancer.And there were statistically interaction between the-592 site A/C polymorphism in IL-10 and H.pylori infection in the occurrence and development process of gastric cancer.
关键词
基因多态性/白介素10/胃肿瘤/幽门螺杆菌/免疫印迹/CagAKey words
polymorphism/IL-10/stomach neoplasms/helicobacter pylori/immunoblotting/CagA引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据