Early growth response gene-1 regulates stathmin gene expression in osteosarcoma Saos-2 cells
AIM: To investigate the effects of stathmin gene expression in osteosarcoma Saos-2 cells induced by early growth response gene-l(Egr1). METHODS: To construct pcDNA3. 1-Egrl recombinant plasmid and then exogenous Egr1 gene was introduced into Saos-2 with Lipofectamine transfection method. The expression of Egr1 gene and stathmin gene were detected by RT-PCR, Western Blot and immunocytochemistry. The apoptosis and proliferation of Saos-2 cells were observed by TUNEL and MTI" assay. RESULTS: The results of RT-PCR indicated that the expression of Egr1 gene was upregulated, however the expression of stathmin gene was downregulated in the transfected cells. MTI" assay revealed that the proliferation of Saos-2 cells transfected EGR-I gene was much lower than that of untransfected cells. The results of TUNEL showed that EGR-1 gene induced apoptosis of the transfected cells. CONCLUSION: Egr1 can suppress the expression of stathmin gene and induce Osteosarcoma Saos-2 cell apoptosis.
NETL
NSTL
万方数据
维普
