Comparison of different dosage of streptozotocin-induced gestational diabetes mellitus on rats
AIM: To explore the effect of the stability of the rat model of gestational diabetes mellitus (GDM) by different dosage of streptozocin(STZ) ,for the research on maternal and fetal GDM provide experimental basis. METHODS: Forty-six Sprague-Dawley pregnant rats were selected and randomly assigned into 4 groups( the low doses group, the medium doses group, the high doses group and the control group). The rats were injected respectively with STZ(35, 45,60 mg/kg) or equal dosage of citrate buffer solution intraperitoneally according to their assigned groups. Their fasting blood glucose were measured after STZ injection 3,9, 14 and 19 d later. During the whole gestation period, the body weights, amount of drinking water, food consumption and urine volume of these rats were tested. Draw a comparison about the modeling rate, turning negative rate and mortality rate in the different groups. The rats in all groups underwent dissection at their gestational ages of 19 d to obtain the pancreatic tissue for pathological study by HE staining. RESULTS: The pregnant rats of the 45 mg/kg group showed significant signs of polyuria, polydipsia, hyperphagia and weight loss after STZ injection, h has the highest making model success rate of 83.3% and the lowest turning negative rate. There was statistically significant difference between the medium doses group and the control group in the level of fasting blood glucose[ (21.8±3.0)vs(5.9±1.2) mmol/L, P<0.05]. The body weight of the medium doses group was also lower than that in control group. The state of hyperglycemia retained longer time and steadily. CONCLUSION: The optimum dose to establish an ideal experimental animal model of streptozotocin-induced GDM is injected STZ 45 mg/kg intraperitoneally with the best stability.
NETL
NSTL
万方数据
维普
