EGFR targeting nanoparticles loading c-erbB2 anti-oligodeoxynucleotides for SK-BR3 human breast cancer cells in vitro
AIM:To investigate the better intake rate of human breast cancer cells SK-BR3 to c-erbB2 anti-oligodeoxynucleotides which was mediated by epidermal growth factor(EGF) coupling of bovine serum albumin (BSA) nanoparticles. METHODS: We used ultrasound emulsification-chemical cross-linking and earboxymethyl reaction to prepare EGF-conjugated albumin targeting nanoparticles. ~(99)mTc marked on the c-erbB2 antioligodeoxynucleotides by paired with Acetyl double-Cysteine. The drug loading rate, encapsulation efficiency and the release rate were detected and calculated of EGF targeting nano-carrier loading c-erbB2 ASODN, and which uptake and stranded rates were explored in SK-BR3 human breast cancer cells. RESULTS:The intake and retention rates in the EGF targeting nano-carrier packeting ~(99)mTc-antisense oligodeoxyribonucleotide ( ASODN ) group were higher than those in the ~(125)I-sense oligodeoxynueleotide (SODN) group and the ~(99)mTc -nonsense oligodenxynucleotide (NSODN) group. At the same time, the uptake of retention rates in the c-erbB2 oligodeoxynucleotide with nano-carrier group were higher than those in the non-nano-carrier group ( P < 0. 05 ). CONCLUSION: ~(99)mTc -EGF-BSA targeting nano-carrier can increase the uptake and retention rates of c-erbB2 anti-oligedeoxy nucleotides in breast cancer SK-BR3 cells, and achieve better targeting effects.
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