Effects of nutrients on blood glucose in STZ diabetic mice
AIM: To observe the effect of different nutrition combinations on blood glucose in STZ diabetic mice and to find the combination which can effectively control blood glucose in type 1 diabetes. METHODS: Mild diabetes mellitus were induced in male KM mice by a single intraperitoneal injection of STZ at a dose of 140 mg/kg. Diabetic mice were assigned randomly to the control group, three treatment groups(group 1,2 and 3) and normal mice were used as the normal group(n =20 in each group). Group 1,2 and 3 were orally administered , respectively, with L-arginine hydrochloride + vitamin A + nicotinamide, taurine + vitamin E + vitamin C + nicotinamide, L-arginine hydroehloride + taurine + biotin + nicotinamide twice daily for 4 weeks. Fasting blood glucose in various groups were measured by an oxidase assay method at 7 th day of nutrition interference. Intraperitoneal glucose tolerance test was performed in the group where their blood glucose were significantly reduced at 28 th day of nutrition interference. The pancreas was removed at 28 th day. Insulun was extracted from pancreas by acid alcohol, and were determined by radioimmunoassay method. Total RNA was extracted from the pancreas by Trizol method. Preproinsulin gene was amplified by RT-PCR. The product was electrophoresed and quantified by image acquisition and analysis system. 13 cell mass was measured from the insulin antibody-stained sections using an image analysis system. PDX-I pritein expression in the pancreas were detected by Western blotting. RESULTS: Fasting blood glucose in group 1 and group 2 were no significant change, and those in group 3 significantly decreased to (6.65 ± 1.34) rranol/L at 7 th day of nutrition interference. The fasting glucose contents in group 3 were respectively (6. 32 ± 1.36), (6. 14 ±1.27), (6. 25 ± 1.43) mmol/L at 14, 21 and 28 thday. The glucose tolerance test shown that the values at points of time of 30 and 120 min in glucose curve were respectively( 13.64 ± 2.05 ), (6.26 ±2.46) mmol/L, which were significantly different from those in the control group. Pancreatic insulin content in group 3 was ( 1.52 ± 0.22) mU/L, which were significantly different from (0. 42 ±0. 11 ) mU/L in the control group. Its preproinsulin mRNA level was 0.8 ± 0.03, and significantly higher than 0. 32 ± 0.02 in the control group. B cell mass in group 3 was (5.11 ±1.56) nag, which were significantly different compaired with(3.45 ± 1.12) mg in the control group. Its PDX-1 protein expression was higher than that in the control group. CONCLUSION: L-arginine hydrochloride + taurine + biotin + nicotinamide can nearly normalize fasting blood glucose and glucose tolerance in STZ diabetic mice through increasing B cell mass and insulin systhesis.
NETL
NSTL
万方数据
维普
