苦参碱对Raji细胞凋亡及凋亡相关蛋白Fas/FasL表达的影响
Raji cell apoptosis-inducing effects of matrine and its effect on Fas/FasL protein expression
刘占术 1陈建斌 1汤为学 2杨泽松 1黄曦 1黄宗干1
作者信息
- 1. 重庆医科大学附属第一医院血液内科,重庆,400016
- 2. 重庆市神经病学重点实验室,重庆,400016
- 折叠
摘要
目的:探讨中药苦参碱对人Burkitt's淋巴瘤Raji细胞凋亡的作用及其可能机制.方法:应用MTT法测定苦参碱对Raji细胞生长的抑制作用;AnnexinV-FITC/PI双染法检测细胞凋亡率;Western Blot检测Fas,FasL,caspase-3蛋白的表达量.结果:苦参碱0.2～1.6 g/L作用Raji细胞24,48,72 h后,对Raji细胞生长具有增殖抑制作用,且呈明显的量效和时效关系,48 h半数抑制浓度(IC_(50))为1.34 s/L.苦参碱0.4,0.8,1.6 s/L组作用48 h后,Raji细胞总凋亡率分别为15.10%,27.88%,48.08%,与对照组8.78%相比较差异具有统计学意义(P<0.05,P<0.01).Western Blot检测结果显示Fas,FasL,caspase-3蛋白表达水平随苦参碱浓度的提高而增加.结论:一定浓度苦参碱可诱导Raji细胞发生凋亡,其凋亡机制可能与上调Fas,FasL蛋白的表达以及激活Caspase-3有关.
Abstract
AIM: To invest the role of matrine-induced apoptosis of Human Burkitt's lymphoma Raji cells and its mechanism. METHODS: The proliferation inhibition rate of Raji cells was performed by MTI' analysis. Cell apoptosis rate was detected by AnnexinV-FITC/PI double staining method. The Fas, FasL and Caspase-3 protein expresssion of Raji cells treated with matrine were evaluated by Western blot. RESULTS: After Raji cells were cocuhured with matrine 0. 2 - 1.6 g/L for 24,48,72 h in vitro, the cell proliferation was inhibited in time-and dosedependent manner, its half maximal inhibitory concentration( IC_(50) )of 48 h was 1.34 g/L. When the Raji cells were cultured for 48 h by matrine 0.4,0. 8,1.6 g/L, the total apoptosis rate 15.10%, 27.88% ,48.08% detected by AnnexinV-FITC/PI double staining, had statistical significance compared with control group 8.78%, ( P < 0.05 or P < 0. 01 ). The protein expresssion levels of Fas, Fasl and Caspase3 increased after Raji cells were treated by matrine 0.4,0. 8,1.6 g/L with a concentration-effect relationship. CONCLUSION : Matrine can induce the apoptosis of Raji cells in vitro, one of its possible mechanisms is that matrine activates caspase-3 through upregulafion of Fas and FasL.
关键词
苦参碱/Raji细胞/Fas/FasL/Caspase-3/凋亡Key words
matrine/Raji cell/Fas/FasL/Caspase-3/apoptosis引用本文复制引用
基金项目
出版年
2009
NETL
NSTL
维普
万方数据