Raji cell apoptosis-inducing effects of matrine and its effect on Fas/FasL protein expression
AIM: To invest the role of matrine-induced apoptosis of Human Burkitt's lymphoma Raji cells and its mechanism. METHODS: The proliferation inhibition rate of Raji cells was performed by MTI' analysis. Cell apoptosis rate was detected by AnnexinV-FITC/PI double staining method. The Fas, FasL and Caspase-3 protein expresssion of Raji cells treated with matrine were evaluated by Western blot. RESULTS: After Raji cells were cocuhured with matrine 0. 2 - 1.6 g/L for 24,48,72 h in vitro, the cell proliferation was inhibited in time-and dosedependent manner, its half maximal inhibitory concentration( IC_(50) )of 48 h was 1.34 g/L. When the Raji cells were cultured for 48 h by matrine 0.4,0. 8,1.6 g/L, the total apoptosis rate 15.10%, 27.88% ,48.08% detected by AnnexinV-FITC/PI double staining, had statistical significance compared with control group 8.78%, ( P < 0.05 or P < 0. 01 ). The protein expresssion levels of Fas, Fasl and Caspase3 increased after Raji cells were treated by matrine 0.4,0. 8,1.6 g/L with a concentration-effect relationship. CONCLUSION : Matrine can induce the apoptosis of Raji cells in vitro, one of its possible mechanisms is that matrine activates caspase-3 through upregulafion of Fas and FasL.
NETL
NSTL
万方数据
维普
