Substance P expression in masseter muscle of infraocclusion rats
AIM: To establish an animal model of infraocclusion, probe deeply into the neurobiologic mechanism of infraocclusion of masseter muscle and provide a new method for prevention and treatment of TMD. METHODS : Fourty-eight rats were divided into 3 experimental groups and 1 control group randomly. Their different part of denture were ground artificially to establish an animal model of infraocclusion. They were group A,ground all denture group,group B, ground posterior denture group, group C, ground anterior denture group, and the control group. The rats of the 4 groups were executed 1 week, 2 weeks and 4 weeks late (n = 4/ group each time). The superficial layer of masseter muscle was harvested to have substance P SABC immunohistochemistry staining, thus to observe the changes of substance P reaction. RESULTS: Substance P SABC immunohistochemistry staining showed, the reaction of substance P in experimental groups was higher than those in control group at every experimental stage( P <0.05), while group A was higher than group C, group C was higher than group B, and in each group, the reation of substance P increased with the extension of the duration of the experiment. CONCLUSION: Substance P had a close relationship with masseter muscle injuries, and substance P may be a partner in the pathological changes of masseter muscle injuries.
NETL
NSTL
万方数据
维普
