Effects of PD K/Akt signaling pathway on vascular endothelial function and interventional effects of erythropoietin
AIM: To investigate the effects of PI3K/Akt signaling pathway on vascular endothelial function in rats with myocardial ischemic postconditioning(I-PostC) and interventional effects of erythropoietin. METHODS: Forty-two male SD rats were randomized into 7 groups: Sham operation group( Sham group) ,ischemia/reperfusion group ( I/R group ), Ischemia-Postconditioning group( I-PostC group) , Wortmannin + I-Post group( Wort + I-PostC group) , rherythropoietin group ( rhEPO group) , Wort + rhEPO group and rhEPO + I-Postc group. Rat models with I/R were induced by ligation of the coronary artery in SD rats. RT-PCR was used to determine Akt mRNA level in each group. Thoracic aortas were harvested and were cut into strips for isolated perfusion experiment on contraction to phenylephrine( PE), endothelium-dependent diastole to acetylcholine(Ach) and non-endothelium-dependent diastole to sodium nitroprusside(SNP). RESULTS: The intervention of I-PcostC and rhEPO could improve strips of thoracic aortas's dilatant reaction to acetylcholine after ischemia/reperfusion injury and increase the expression of Akt mRNA level in the aortas. There's no significant statistical difference in effects of the two methods above. Wort, the inhibitor of PI3K,eould impair the effects; Combined use of I-PcostC and rhEPO showed no statistical difference compared with only use I-PcostC or rhEPO. CONCLUSION: I-PostC and rhEPO can play a role in protecting vascular endothelial function via PI3K/Akt signal transduction pathway.
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NSTL
万方数据
维普
