Effect of DNAzyme on expression of LMP1 gene on human nasopharyngeal carcinoma xenograft in nude mice
AIM: To observe the inhibitory effect of DNAzyme targeting on Epstain-Barr virus encoded latent membrane protein 1 (EBV-LMP1) on the growth of nasopharyngeal carcinoma ( NPC) xenograft in nude mice. METHODS: We designed and synthesized DNAzyme ( DZ509 ) , mutant DNAzyme ( mutDZ509 ) and antisense oligonucleotides ( ASODN509) targeting on EBV-LMP1; nasopharyngeal carcinoma cell (C666-1 cell) was injected into 28 nude mice subcutaneously to establish cancer model and then divided into 4 groups including DZ509, mutDZ509, ASODN509 and PBS. We described growth curve of tumor, tumors were cutting after 8 d, detected LMP1 mRNA by reverse transcription-PCR (RT-PCR), and LMP1 by immunohistochemistry and Western Blot. RESULTS: NPC cancer models with nude mice were established; Among DZ509,mutDZ509,ASODN509 and PBS, the growth velocity of tumor stepped down, the rate is 85. 53% ,73. 03% , 52.77%,and the expression of LMP1 mRNA and LMP1 protein increased in turn. CONCLUSION; Injecting DNAzyme into tumors in vivo can inhibit the express of LMP1 and growth of nasopharyngeal carcinoma xenograft effectively. DNAzyme targeting on LMP1 may be a new promising gene therapy for NPC.
NETL
NSTL
万方数据
维普
