Inhibitory effects of FAK on cellular proliferation and apoptosis of nasopharyngeal carcinoma cells
AIM: To investigate the inhibition effects of FAK(focal adhesion kinase ) with RNA interference ( RNAi) on cellular proliferation and apoptosis of human nasopharyngeal carcinoma (NPC) HNE-1 cells. METHODS: The expression of FAK in HNE-1 cells was detected by immunohistochemical staining. According to FAK mRNA sequence at the GenBank, the siRNA against FAK were designed and transfected into HNE-1 cells. The expression levels of FAK were confirmed by RT-PCR and Western Blot after the total RNA and protein were collected. The proliferation of HNE-1 cells were determined by MTT assay and the cell cycle and apoptosis were examined by flow cytometry ( FCM). RESULTS: The expression of FAK in HNE-1 cells was positive. The siRNA against FAK downregulated the expression level of mRNA and protein dramatically in HNE-1 cell line, with a down-regulation of 71.4% and 59. 8%. The result of MTT indicated that the cancer cellular proliferation was restrained remarkably and the inhibition ratio was 36. 8%. At 48 h after the transfection, the number of HNE-1 cells in the G0/G1 phase was increased, that in the S and G2-M phases were depressed. Meanwhile, the apoptosis was increased. CONCLUSION; To interfere expression of FAK in HNE-1 cells, HNE-1 cell proliferation can be inhibited, the cell cycle was induced to redistribute, and so was the cell apoptosis.
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