Effect of mongolian milkvetch root and its compound on chronic atrophic gastritis rats
AIM: To explore the treatment effectiveness and action mechanism of mongolian milkvetch root and its compound on chronic atrophic gastritis(CAG) rats. METHODS: Eighty Wistar rats, half male and half female, were randomized into normal group (n = 12 ) and model group ( n = 68). Nomal group were fed by common diet and free drinking; the CAG model were made by complex method in model group. After 23 weeks, the model were made successfully. Then the model group were randomized into 6 groups: model group ( n = 8 ) , single mongolian milkvetch root group (n = 8) , high-dose combined group ( n = 8) , middle-dose combined group ( n = 8 ) , low-dose conbined group ( n = 8 ) and folic acid treating group. All treatment groups were treated for 7 weeks. All rats were observed in their histopathological changes under a light microscope with HE staining and detected proliferating cell nuclear antigen ( PCNA ) , B-cell leukemia/lymphoma-2 ( BCL-2 ) , Interleukin-4 ( XL-A ) , interferon gamma ( IFN-γ ) , Transforming growth factor betal ( TGF-01 ) with elasia method. RESULTS: Compared with the normal group, PCNA (153. 08 ± 38.67) ,BCL-2( 124. 90 ±20. 79) , IFN-γ(27. 99 ±5. 57) ,TGF-βl (228. 97 ± 79. 50) of the model group increased(P < 0. 05); compared with the model group, PCNA and BCL-2 of the the single mongolian milkvetch root group (48. 83 ± 12.45), (73.05 ± 20. 22), high-dose combined group (101. 18 ± 31. 88), ( 97. 72 ± 25. 34), middle-dose combined group(69. 03 ± 13. 91), (78. 35 ± 15. 95) , low-dose conbined group (72. 32 ± 19. 15 ) , ( 84. 58 ± 19.66 ) decreased (P <0.05); IFN-γ of the single mongolian milkvetch root group( 15.79 ± 2. 68 ) , middle-dose combined group ( 17. 04 ± 6. 92 ) ,low-dose conbined group (17. 02 ± 6. 35) decreased ( P < 0. 05 ). CONCLUSION: Mongolian milkvetch root and its compound by regulating cell proliferation and apoptosis of dynamic equilibrium and the treatment of immune function in rats with chronic atrophic gastritis.
NETL
NSTL
万方数据
维普
