基质金属蛋白酶13和转化生长因子β1 mRNA及蛋白在骨性关节炎中的表达
Expression of MMP-13 mRNA, TGF-β1 mRNA and proteins in osteoarthritis
郭静 1李琪佳1
作者信息
- 1. 华北煤炭医学院实验中心,河北,唐山,063000
- 折叠
摘要
目的:探讨骨性关节炎(OA)中金属蛋白酶-13(MMP-13),转化生长因子-β1(TGF-β1)mRNA和蛋白表达的意义.方法:采用免疫组织化学技术,对60例OA病例及20例正常对照组进行了MMP-13及TGF-β1蛋白表达的检测;通过原位杂交技术,对30例OA及1O例正常对照进行了MMP-13及TGF-β1在mRNA水平表达的检测.结果:MMP-13mRNA和蛋白在OA组阳性表达的积分光密度(IOD)值均显著高于正常对照组(P<0.01),OA中两耆的表达有显著正相关性(P<0.01);TGF-β1 mRNA和蛋白在OA组阳性表达的IOD值均显著高于正常对照组(P<0.01),OA中两者的表达亦有显著正相关性(P<0.01);MMP-13 mRNA,TGF-β1mRNA及相应蛋白在OA中的表达均呈负相关(P<0.05或P<0.01).结论:OA中TGF一β1高表达通过下调关节软骨与滑膜细胞中MMP-13的表达,对OA关节软骨起保护作用.
Abstract
AIM: To investigate the significance of expression of MMP-13, TGF-βl mRNA and corresponding proteins in Osteoarthritis. METHODS: Immunohistochemical ( IHC) analysis was used to determine the distribution of MMP-13 and TGF-βl protein in chondroeytes and synoviocytes of osteoarthritis from 60 patients with osteoarthritis group and 20 patients with normal control group. Expression of MMP-13 and TGF-βl mRNA in chondric and synovial tissues was detected in 30 cases with osteoarthritis group and 10 cases with normal control group by in situ hybridization(ISH). RESULTS: IOD of MMP-13 mRNA and protein in OA was significantly higher than normal control group ( P < 0.01), and there was significant difference between IOD of TGFβ1 mRNA and protein in OA and control group ( P < 0. 01 ). There were positive correlations both between mRNA and corresponding protein in OA ( r = 0. 956, 0. 941, P < 0. 01 ). There were also significantly negative correlations both between IOD of MMP-13 and TGF-pl in OA detected by ISH and IHC ( r = -0. 385 , P < 0. 05; r = - 0. 603 , P < 0. 01 ) , respectively. CONCLUSION: The higher expression of TGF-pl may be protect articular cartilage by downregulation the expression of MMP-13 of chondroeytes and synoviocytes in OA.
关键词
骨性关节炎/基质金属蛋白酶-13/转化生长因子-β1/原位杂交/免疫组织化学Key words
osteoarthritis/MMP-13/TGF-β1/in situ hybrid ization/immunohistochemistry引用本文复制引用
基金项目
河北省教育厅科学研究计划项目(2005105)
出版年
2009
NETL
NSTL
维普
万方数据