Expression of MMP-13 mRNA, TGF-β1 mRNA and proteins in osteoarthritis
AIM: To investigate the significance of expression of MMP-13, TGF-βl mRNA and corresponding proteins in Osteoarthritis. METHODS: Immunohistochemical ( IHC) analysis was used to determine the distribution of MMP-13 and TGF-βl protein in chondroeytes and synoviocytes of osteoarthritis from 60 patients with osteoarthritis group and 20 patients with normal control group. Expression of MMP-13 and TGF-βl mRNA in chondric and synovial tissues was detected in 30 cases with osteoarthritis group and 10 cases with normal control group by in situ hybridization(ISH). RESULTS: IOD of MMP-13 mRNA and protein in OA was significantly higher than normal control group ( P < 0.01), and there was significant difference between IOD of TGFβ1 mRNA and protein in OA and control group ( P < 0. 01 ). There were positive correlations both between mRNA and corresponding protein in OA ( r = 0. 956, 0. 941, P < 0. 01 ). There were also significantly negative correlations both between IOD of MMP-13 and TGF-pl in OA detected by ISH and IHC ( r = -0. 385 , P < 0. 05; r = - 0. 603 , P < 0. 01 ) , respectively. CONCLUSION: The higher expression of TGF-pl may be protect articular cartilage by downregulation the expression of MMP-13 of chondroeytes and synoviocytes in OA.
NETL
NSTL
万方数据
维普
