Expression of centrosome-related KinasesNek2 in gastric carcinoma and their significances
AIM: To analyze its effect on the invasion of gastric cancer and to find a new target for clinical diagnosis and treatment of gastric cancer by investigating the expression of centrosomerelated kinase-Nek2 in gastric cancer. METHODS: Gastric cancer tissues and tissues in the surgical margin were collected from 50 patients who underwent surgery in our hospital. Real time quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry were used to detect the expression of Nek2 in these samples. The correlation between the expression of Nek2 and patients' clinicopathologic parameters were analyzed. RESULTS: The expression of Nek2 mRNA and protein in gastric cancer were significantly higher than that in tissues in the surgical margin(P <0. 05). There was significant correlation between the expression of Nek2 mRNA and protein in gastric cancer and TNM stage, depth of primary tumor invasion, differentiation and lymph node metastasis(P<0.05). CONCLUSION: The Nek2 is over expressed in gastric cancer at mRNA and protein levels, and posi tively correlated with the depth of primary tumor invasion, differ entiation, lymphatic node metastasis as well as TNM stage of gas tric cancer, which indicates that Nek2 promotes tumor progression to some degree. Nek2 can be used to determine the extent of progress and prognosis of gastric cancer.
NETL
NSTL
万方数据
维普
