纤维蛋白原Bβ-249C/T和Bcl-1G/A基因多态性与脑梗死类型的相关性
Research on association of beta-fibrinogen gene-249C/T and Bcl-1G/A polymorphisms and type of cerebral infarction
杨娜 1元小冬 1许亚茹 2李宏芬 1王淑娟1
作者信息
- 1. 华北煤炭医学院,附属开滦医院神经内科河北,唐山,063000
- 2. 华北煤炭医学院,附属医院院内感染科,河北,唐山,063000
- 折叠
摘要
目的:研究纤维蛋白原(Fg)Bβ-249C/T,Bcl-1G/A的基因多态性、血浆Fg浓度、分子聚合活性等指标与脑梗死(CI)类型的关系.方法:采用病例对照研究方法,选取主干支脑梗死(MCI)患者54例,穿通支脑梗死(PCI)患者106例,正常对照组160例,应用聚合酶链反应-限制性酶切法进行Bβ-249C/T,Bcl-1G/A位点的基因多态性分析.抽取患者清晨空腹静脉血测定血糖等4项生化指标和血浆Fg浓度,Fg单体聚合反应速率(FMPV),最大光密度(Amax),FMPV/Amax等反映Fg分子聚合功能的参数,并进行体检.结果:BβBecl-1G/A的A等位基因和GA,AA基因型在MCI组均高于财照组(P<0.05),FgBβ-249C/T等位基因和各基因型在MCI组,PCI组和对照组组间的分布频率差异无显著性.MCI组Bβ-249C/T的CT+TT型Fg浓度(4.04 4±0.88)g/L,FMPV(0.72±0.12)均低于cc型Fg浓度(5.Ol±0.43)g/L,FMPV(0.83±0.06)(P<0.05).对照组B13Bcl-1G/A的GA+从型Fg浓度(4.03±0.90)s/L高于GG型(3.69 4±0.95)g/L(P<0.05);PCI组B13Bcl.1G/A的GA+AA型FMPV(0.74±0.11)高于GG型(O.69±0.11)(P<0.05).结论:B13-249C/T基因多态性可能是影响血浆FMPV功能表达的重要位点,BβBcl-lGA,AA基因型可能仅使血浆纤维蛋白单体聚合功能表达增强而诱发PCI.
Abstract
AIM: To explore the relation of Beta-fibrinogen( Fg) gene-249C/T and Bcl-IG/A polymorphisms, the Fg indexes of Fg concentration, molecular polymerized function and the type of cerebral infarction. METHODS: A case-control study was used to analyze 54 patients with main-trunk cerebral infarction( MCI) , 106 patients with penetrating cerebral infarction ( PCI) and 160 normal control; Gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism; All sample with empty stomach should be drawn venous blood on early morning to determine 4 biochemistry indexes and the parameters illustrated Fg molecular reactivity, such as: Fg concentration, fibrin monomer polymerize velocity ( FMPV ) , absorbance maximum ( Amax ) , FMPV/Amax; All sample were examined by trained interviewers. RESULTS: The frequency of A Bcl-1 allele, GA + AA genotype in the group of MCI were higher than that of normal control (P < 0. 05) , No distinction of the frequency of BB-249C/T's genotype and allele between MCI, PCI and normal control were found. In the group of MCI, Fg concentration and FMPV of BS-249C/T CT + TT genotype were (4. 04 ± 0. 88 ) g/L and ( 0. 72 ±0. 12 ) by turns, while these of CC genotype were(5. 01 ± 0. 43 ) g/L and (0. 83 ±0.06). Fg concentration and FMPV of BB-249C/T CT + TT genotype was lower than that of CC genotype ( P < 0. 05 ). In the group of normal control, Fg concentration of BBBcl-lG/A GA + AA genotype was(4. 03 ±0. 90) g/L, which was higher than GG genotype's Fg concentration that was ( 3. 69 ± 0. 95 ) g/L ( P < 0.05). In the group of PCI, FMPV of BBBcl-1 G/A GA + AA geno-type was(0.74 ±0.11) , which was higher than GG genotype's FMPV that was(0.69 ±0.11) (P < 0.05). CONCLUSION: BB-249C/T was probably the important sites of the function express of FMPV, BβBcl-1GA, AA genotype could induce PCI by increasing the function expression of fibrin monomer polymerize function.
关键词
纤维蛋白原/基因多态性/Bβ-249C/T/BβBcl-1G/A/脑梗死类型Key words
fibrinogen/gene polymorphisms/BB-249C/T/BBBcl 1G/A/cerebral infarction type引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据