Effect of antioxidants on NK activity and chemiluminescence of umbilical blood
AIM: To investigate the effect of antioxidants, melatonin, sodium selenite, N-acetyl-cysteine(NAC) and quercetin, on natural killer cell activity(NK activity) and chemiluminescence (CL) of umbilical blood. METHODS: Methlthiazolyl tetrazolium (MTT) method and CL assay were used to detect NK activity and CL intensity during the process of killing target cell. RESULTS: 1 - 100 μmol/L melatonin and 0.43 -6.96 μmol/L sodium selenite significantly increased NK activity. CL intensity was greatly enhanced by 10 μmol/L melatonin, 0. 87 -3.48 μmol/L sodium selenite and 6.25 μmol/L quercetin. All the antioxidants delayed the time of CL. Melatonin and quercetin made the CL showed clearly double peaks. CONCLUSION: Antioxidants have the ability to modulate immune function of NK cell and this maybe related to the oxidative state of neonate at birth.
NETL
NSTL
万方数据
维普
