Correlation of activation of T cell signal molecular expression and colon cancer cell apoptosis
AIM: To investigate the correlation of activation of T cell combined with CpG ODN signal molecular expression and colon cancer cell apoptosis. METHODS: Cell phenotype in PBLs were by the flowcytometry. The microchange of the tumor ceils was investigated by electron microscope and the apoptotic index of the colon carcinoma cell was detected by the flow cytometry. RESULTS: Expression of CD28 in stimulated PBLs was a little more than those without stimulation. Cell expression of CD3, CD4 in stimulated PBLs was significantly more than those without stimu lation. Electron microscope verified that tumor cell necrosis occurs after 48 h exposion to costimulate activation of PBLs. Flow cytometer verified that cells apoptosis after 72 h exposion to costimulate activation of PBLs. CD3 and CD8 increased in tumor patients PBLs being costimulating combined with CpG ODN (P < 0. 05). CONCLUSION: Costimulating signal molecular increased, cyto kine and killer cell secreted more when activation of T cell com bined with CpG ODN. The colorectal carcinoma cell apoptosis was bring by these factors. CD3 and CD8 increased in tumor patients PBLs being costimulating combined with CpG ODN. Correlation of activation of T cell combined with CpG ODN signal molecular expression and colon cancer cell apoptosis was shown by the re sults.
T lymph cellscostimulationmembrane molecularcolon cancer cellapoptosis
NETL
NSTL
万方数据
维普
