Phenylpropanoid glycosides reversing effect on multidrug resistance of colon carcinoma LoVo/Adr cells
AIM: To investigate the effect of phenylpropanoid glycosides ( PPG) on reversing multidrug resistance ( MDR) of colon carcinoma LoVo/Adr cells and its possible mechanism. METHODS: The effects of PPG on proliferation of LoVo and LoVo/ Adr cells were examined by MTT. The reversing test of PPG on MDR was identified when its IC10 was < 10% ; its IC_50, and rever sal fold was subsequently calculated. Flow cytometry technique was applied to determine the influence of PPG on the uptake of ad riamycin( ADR). The subcellular distribution of ADR in the cells was observed by fluorescence microscopy. RESULTS: At a con centration of 80 and 160 mg/L of PPG, the survival rate of LoVo cells was(69.34 ±3.48)% and(30. 15 ± 1. 86)% , respectively; while the survival rate of LoVo/Adr cells was (67. 89 ±2. 87) % and(34.96 ±2. 14)% , respectively. Therefore, PPG significantly inhibited proliferation of cells in a dose-dependent manner ( P < 0. 05) . However, when the concentration of PPG was less than 40 mg/L, the survival rate of LoVo cells as well as LoVo/Adr cells was more than 90% . Reversal fold was 5. 24 and 9. 78 corresponding to a concentration of 20 and 40 mg/L of PPG, respectively. Reversal fold was also dose-dependent. The distribution of fluorescent ADR was predominantly localized in the nucleus, and the intensity of ADR fluorescence was significantly increased from (2. 04 ± 0. 70) to( 2. 35 ± 0. 87 ) ( P < 0. 05 ) , after treatment with PPG. CONCLUSION: PPG significantly inhibits carcinoma and reverses multidrug resistance of LoVo/Adr cells. By increasing the uptake of ADR and preferably accumulating ADR in nuclei of LoVo/Adr cells is probably one of mechanisms to explain the reversing effect of PPG on MDR.
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万方数据
维普
