Proteomic research of mouse's substantia nigra of chronic Parkinson's disease induced by rotenone
AIM: To search for the DSPs of PD,we separate and evaluate rotenone-induced differential protein expression through the use of proteomics in the Substantia nigra of a mouse model of chronic Parkinson's disease. METHODS: Establishing Parkinson's mouse model induced by rotenone, proteins of control and treated are extracted, and 2-DE handbook ( Bio-Rad Company ) was refer-enced for two-dimensional electrophoresis. PDQUEST8.0 analytical electrophoresis pattern was adopted to evaluate differential protein expression. The altered protein are identified with MALDI-TOF-MS and database searching. RESULTS: Thirty-two differential protein expressions between the two groups. One is the same result of our study teams, another new protein is evaluated.CONCLUSION:There are two proteins that exhibit differential expression,Coiled-coil domain-containing protein 85A, Ubiquitin-like protein 3 precursor. The changes of these proteins are probably related with the DSPs of PD.
NETL
NSTL
万方数据
维普
