Roux-en-Y胃旁路术改善非肥胖型糖尿病大鼠糖脂代谢
Effects of gastric bypass on carbohydrate and lipid metabolism in non-obese diabetic Goto-Kakizaki rats
李桢 1李东飞 1张红亚 2梁炜 2戴景兴 1原林1
作者信息
- 1. 南方医科大学基础医学院临床解剖学研究所国家级重点学科,广东广州510515
- 2. 新乡医学院第二附属医院,河南省精神生物医学重点实验室,河南新乡453002
- 折叠
摘要
目的:观察Roux-en-Y胃旁路术对非肥胖糖尿病Goto-Kakizaki(GK)大鼠血糖和血脂代谢的影响.方法:18只GK大鼠随机等分为Roux-en.Y胃旁路手术组(RYGB组)、假RYGB组和对照组;观察手术后1,2,4,12 wk各组血清葡萄糖和胰岛素变化,观察手术后4,12 wk血清总胆固醇、三酰甘油和游离脂肪酸含量以及口服葡萄糖耐量试验和口服葡萄糖后胰岛素变化.结果:手术后各组摄食量无差异(F=1.222,P=0.322);手术后RYGB组空腹血糖较假RYGB组和对照组明显下降(F=10.064,P=0.002);RYGB组空腹胰岛素较假RYGB组和对照组明显升高(F=56.138,P=0.000).手术后RYGB组4,12 wk口服葡萄糖耐量试验血糖值曲线下面积明显减少(F=4.738,P=0.016),与假RYGB组和对照组比较均存在统计学差异(P=0.039,P:0.028);胰岛素值曲线下面积明显增加(F_(4wk)=192.112,P=0.000;F_(12wk)=228.284,P=0.000).RYGB组术后血清总胆固醇显著下降(P<0.05);血清三酰甘油和游离脂肪酸在RYGB组均明显降低(P<0.05).结论:ROtLX-en-Y胃旁路术可改善非肥胖糖尿病GK大鼠血糖、血脂代谢及胰岛功能,且与摄食量无关.
Abstract
AIM: To observe the effects of gastric bypass on carbohydrate and lipid metabolism in non-obese diabetic Goto-Kakizaki (GK) rate. METHODS: Eighteen male GK rats were randomly allocated into 3 groups; Roux-en-Y gastric bypass (RYGB) group, sham RYGB group (sham-RYGB) and pair-fed control group. Each group was monitored for fasting glucose, oral glucose tolerance and insulin as well as plasma levels of cholesterol, triglycerides, and free fatty acids on 1st, 2nd, 4th, 12th weeks, post-operatively. RESULTS: No significant differences were observed in food intake among all groups ( F = 1. 222, P = 0.322). Fasting blood glucose levels measured post operatively showed significant drop in RYGB group compared to two control groups (F = 10.064, P = 0.002 ) , and significant elevation of fasting insulin(F = 56.138,P =0.000) in RYGB group. On post-operative 4th and 12th weeks, OGTT performed showed significant reduction of the area under the curve ( AUC) of serum glucose ( F =4.738, P = 0. 016) ,and statistical difference was observed in comparison to control groups ( P = 0. 039, P = 0.028 ). Significant increase was observed in the area under the curve (AUC) of serum insulin ( F_(4 wk) =192. 112,P =0. 000; F_(12wk)=228. 284,P = 0.000). Post operative values of cholesterol, triglycerides, and free fatty acids in RYGB group were significantly lower than that of sham-RYGB and control group( P < 0.05 ). CONCLUSION: Roux-en-Y gastric bypass is very effective in down-regulation ofthe carbohydrate and lipid metabolism through the enhancement of insulin function in non-obese diabetic GK rats, and these effects are not associated with weight loss or lower food intake.
关键词
胃旁路术/2型糖尿病/动物/糖代谢/脂质代谢Key words
gastric bypass/2 type diabetes mellitus/animals/carbohydrate metabolism, lipid metabolism引用本文复制引用
基金项目
国家重点基础研究发展规划(973计划)(2007CB512700)
出版年
2009
NETL
NSTL
维普
万方数据