PUMA在小鼠肝再生早期的表达及其调控机制
Expression and underlying regulation of PUMA during liver regeneration in mice
陈松 1郝强 1杨生生 1陈欢 1焦炳华 1蔡在龙1
作者信息
- 1. 第二军医大学基础部生物化学与分子生物学教研室,上海200433
- 折叠
摘要
目的:观察部分肝脏切除术后PUMA及其相关的转录因子在小鼠肝再生早期的表达.方法:成年雄性C57/BL6小鼠经70%肝脏切除后建立肝再生模型,采用实时定量PCR,Western印迹和免疫组化方法研究PUMA在肝再生不同时期(共7个时间点)的表达,同时还检测了p53和Slug蛋白水平的变化.结果:同对照组相比,PUMA mRNA自肝切术后6-72 h显著下降(P<0.05),同其蛋白水平的变化一致;同时p53和Slug蛋白分别自术后24 h和6 h上调.结论:在肝切术后72 h,PUMA的表达下调并不依赖p53,可能与Slug的上调相关,提示PUMA在肝再生早期可能参与对细胞凋亡的调节.
Abstract
AIM: To study expression and underlying regulation factor of PUMA during the early phase of liver regeneration in mice. METHODS: liver regeneration model was produced by 70% partial hepatectomy ( PH) in male C57/BL6 mice. Then the mice were sacrificed and the remanent livers were prepared. Hepatic PUMA expression were assessed at 7 different time points during the liver regeneration using real-time PCR, Western Blot and immunohistochemistry. Hepatic levels of p53 and Slug protein were also determined in this process by Western Blot. RESULTS: Hepatic PUMA mRNA decreased significantly 6-72 h after PH compared with that of control group ( P < 0. 05 ) , which was coupled with the change of PUMA protein level. Hepatic p53 and Slug protein levels increased from 24 and 6 h after PH, respectively. CONCLUSION:During 72 h after PH in mice, PUMA repression was independent of p53, but might be associated with Slug, which PUMA may play a role in regulation of apoptosis in liver regeneration.
关键词
肝再生/部分肝脏切除术/PUMA/p53/SlugKey words
liver regeneration/partial hepatectomy/PUMA/p53引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据