Expression and underlying regulation of PUMA during liver regeneration in mice
AIM: To study expression and underlying regulation factor of PUMA during the early phase of liver regeneration in mice. METHODS: liver regeneration model was produced by 70% partial hepatectomy ( PH) in male C57/BL6 mice. Then the mice were sacrificed and the remanent livers were prepared. Hepatic PUMA expression were assessed at 7 different time points during the liver regeneration using real-time PCR, Western Blot and immunohistochemistry. Hepatic levels of p53 and Slug protein were also determined in this process by Western Blot. RESULTS: Hepatic PUMA mRNA decreased significantly 6-72 h after PH compared with that of control group ( P < 0. 05 ) , which was coupled with the change of PUMA protein level. Hepatic p53 and Slug protein levels increased from 24 and 6 h after PH, respectively. CONCLUSION:During 72 h after PH in mice, PUMA repression was independent of p53, but might be associated with Slug, which PUMA may play a role in regulation of apoptosis in liver regeneration.
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万方数据
维普
