Effects of angelica polysaccharides on expression of adhesion molecules and adhesive function of murine bone marrow and peripheral blood mononuclear cells
AIM: To explore the effects of angelica polysaccharides ( APS) on the expression of adhesion molecule and adhesive function in murine bone marrow and peripheral blood mononuclear cells (MNC), so as to provide experimental evidence of APS on hematopoietic regulation and the mechanism of mobilizing. METHODS: The effects of APS on expression of CD49d and CD44 in murine bone marrow and peripheral blood MNC were analyzed by flow cytometry; the effects of APS on adhesive function in vitro and in vivo were evaluated by MTT assay. RESULTS: In APS group (4 mg/kg) : the percent of CD49d' expression on murine bone marrow and peripheral blood MNC obviously decreased ( P < 0. 01 ) , and the percent of CD44' expression on bone marrow MNC also decreased ( P < 0.05 ) , but we did not observe any change of CD44' expression on bone marrow MNC (P>0. 05); In APS group (4 mg/kg) : adhesive function of bone marrow and peripheral blood MNC to fibronectin ( FN) was inferior to control group ( P < 0. 01 ) , but APS group did not have significant change with rhG-CSF group ( P > 0.05 ) ; With APS in vitro, the adhesive function of bone marrow and peripheral blood MNC to FN decreased in all the groups (without 50 |xg/mL APS group) , APS groups of the same concentration did not have any change at different times (P >0.05). CONCLUSION: APS can decrease the expression of CD49d and CD44 in murine bone marrow and peripheral blood MNC, adhesive function in vitro and in vivo also decreased.
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维普
