Reparation of bone marrow mesenchymal stem cell-transplantation for the ulcerative colitis rat
AIM: To investigate the reparation of bone marrow mesenchymal stem cell-transplantation for the ulcerative colitis rat. METHODS: The BMSC of SD rat were isolated, cultured and labelled with fluor. SD rats were randomly distributed into 4 groups, Colitis was induced with immune-combined TNBS/ethanol in group A, B and C, but was not induced in group D. Following the induction of colitis, each rat in 4 groups received caudal vein injection of 1 mL fluids, which were: normal sodium for group A, BMSCs for group B and D, BMSCs and ECF for group C. Five rats in each group were sacrificed at day 3, 7 and 14 after injection. The distribution of BMSCs in colon was observed with fluorescent microscope; the pathological-changes and expression of TNF-α and IL-10 in colon were observed, too. RESULTS: The fluor marked BMSCs could be seen in the colic tnucosa in each group on day 3, and there were more BMSCs in the colonic focus of infection than the normal mucosa. The numbers of BMSCs in the colon of group B and C were more than those in group D( P < 0.05). Compared with those of group A, there was a higher expression of IL-10 and a lower expression of TNF-a in colonic mucosa of group B and C. The reparation of injuried colonic mucosa in group B and C were obvious surpassed than that in group A, but there was no obviously difference between group B and C. CONCLUSION: Transplantation of BMSCs could promote the reparation of impaired colonic mucosa in UC rat. EGF injected one time could not promote BMSCs migranting to the injured colonic tissue and repairing the damage of colon in UC rat.
bone marrow mesenchymal stem cellstransplantationulcerative colitis
NETL
NSTL
万方数据
维普
