Cisplatin augments apoptosis of esophageal carcinoma cells induced by agonistic anti-hDR5 antibody by DR5 and mitochondria death pathway
AIM: To probe into the effect of cisplatin in combination with agonistic anti-hDR5 antibody mDRA-6 on apoptosis of human esophageal carcinoma cell line EC9706 and its mechanisms. METHODS:The cytotoxic action of mAb mDRA-6 and cisplatin on EC9706 cells, DR5 expression on EC9706 cell surfaces, apoptosis of EC9706 cells. Mitochondrial membrane potential and reactive oxygen species ( ROS) were assessed by flow cytometry. The morphological changes and DRS expression of EC9706 cells were observed by fluorencence microscope. RESULTS: mDRA-6 or cicplatin exerted cytotoxicity on EC9706 cells 109 cells and activates mitochondrial apotosis pathway by up-regulation of ROS so that cisplatin acts synergisti-cally to induce apoptosis of EC9706 cells in combination with agonistic anti-hDR5 antibody. The results maybe provide potential instruction significance for clinical application of agonistic anti-hDR5 antibody and rTRAIL.
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