兔曲霉菌性角膜炎共焦显微镜与组织病理检查的对比分析
Contrastive analysis of confocal microscopy and histopathologic with the rabbit fungal (aspergillus) keratitis
杜蕊 1吴洁 1马吉献 2朱秀萍 1刘先宁 1程燕 1阎艳3
作者信息
- 1. 陕西省眼科研究所,西安市眼科医院,陕西西安710002
- 2. 第四军医大学西京医院眼科,陕西西安710033,
- 3. 西安市第一医院,陕西西安710002
- 折叠
摘要
目的:探讨共焦显微镜与组织病理检查对真菌性角膜炎的诊断价值.方法:选择有色实验兔24只建立曲霉菌性角膜炎的模型,分别在接种后的第3,7,10,15日行角膜共焦显微镜与组织病理检查.结果:曲霉菌接种后的第3,7,10日角膜共焦显微镜与组织病理切片(HE,PAS染色)检查均可见曲霉菌菌丝及孢子,呈垂直、斜形生长,周围伴有较多炎性细胞(中性粒细胞、巨噬细胞)浸润,第15日角膜共焦显微镜可见到少量稀疏分布、短小的真菌菌丝.组织病理切片未见明显菌丝生长.结论:角膜共焦显微镜与组织病理对曲霉菌菌丝、孢子及生长方式的检测一致.角膜共焦显微镜是一种无创、快速的检查方法,为真菌性角膜炎的早期诊断、治疗用药、手术时机及手术方式的选择提供重要客观的依据.
Abstract
AIM: To evaluate the value of confocal mictoscopy and histopathological examination in diagnosis of fungal keratitis. METHODS:Twenty-four pigmentary rabbit fungal keratitis model of Aspergillus were built. Confocal microscopy and histopatholo-gial examination were used in 3, 7, 10 and 15 d after aspergillus was inoculated. RESULTS: Aspergillus hypha, spore and inflammatory infiltrates were found by confocal microscopy and his-topathologial in 3, 7, 10 d after aspergillus was inoculated. Most Aspergillus hypha grew in cornea perpendicularly and obliquely. In the anaphase (15 d after aspergillus injection) , a little hypha was found by confocal microscopy while it could not be found by pathological section examination. CONCLUSION: The outcome of Aspergillus hypha, spore and kinds of fungi grew in cornea by confocal microscopy was consistent with histopathological characteristics. Confocal Microscopy provides a means of no wound and fast for clinical diagnosis of fungal keratitis.
关键词
实验兔/曲霉菌/角膜共焦显微镜/病理Key words
experimental rabbit/aspergillus/confocal microscopy/pathological引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据