Effect of small interference RNA on expression of the Skp2 in Eca-109 cell line
AIM: To study the expression of Skp2 mRNA and the proliferation activity of Eca-109 cell line after transfected Skp2 siRNA. METHODS: The Skp2 siRNA chain were synthesized and transfected into Eca-109 cell line by RNA-Mate reagent. RT-PCR was used to analyzed the quantity of Skp2 mRNA, CCK-8 was used to detect the proliferation activity of Eca-109 cell line. RESULTS: Significant down-regulation of the Skp2 mRNA were found following transfection of the Skp2 siRNA chain, and the proliferation activity of Eca-109 cell line was inhibited. CONCLUSION: The transfection of synthesized Skp2 siRNA in Eca-109 cell line can down-regulate the expression of Skp2 mRNA. As an important tool of the gene silencing siRNA is possibly to be a new method of treating the esophageal cancer.
NETL
NSTL
万方数据
维普
