siRNA干扰对Eca-109食管癌细胞株Skp2表达的影响
Effect of small interference RNA on expression of the Skp2 in Eca-109 cell line
宋旭东 1徐洪1
作者信息
- 1. 华北煤炭医学院病理学教研室,河北唐山063000
- 折叠
摘要
目的:探讨siRNA干扰后食管癌Eca-109细胞株skp2 mRNA表达及肿瘤细胞增殖活性的影响.方法:通过siRNA转染食管癌Eca-109细胞株后,分别用RT-PCR和CCK-8检测skp2 mRNA的表达和细胞增殖的活性.结果:食管癌Eca-109细胞株增殖活性及Skp2 mRNA明显减少.结论:运用siRNA可以有效抑制skp2 mRNA的表达及细胞增殖活性.siRNA有望成为治疗食管癌的重要手段.
Abstract
AIM: To study the expression of Skp2 mRNA and the proliferation activity of Eca-109 cell line after transfected Skp2 siRNA. METHODS: The Skp2 siRNA chain were synthesized and transfected into Eca-109 cell line by RNA-Mate reagent. RT-PCR was used to analyzed the quantity of Skp2 mRNA, CCK-8 was used to detect the proliferation activity of Eca-109 cell line. RESULTS: Significant down-regulation of the Skp2 mRNA were found following transfection of the Skp2 siRNA chain, and the proliferation activity of Eca-109 cell line was inhibited. CONCLUSION: The transfection of synthesized Skp2 siRNA in Eca-109 cell line can down-regulate the expression of Skp2 mRNA. As an important tool of the gene silencing siRNA is possibly to be a new method of treating the esophageal cancer.
关键词
skp2/小干扰RNA/食管癌Key words
Skp2/siRNA/esophageal cancer引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据