Bc1-2和Bax在臀肌挛缩带中的表达及其对挛缩带形成的作用
Expression and effect of Bcl-2 and Bax on gluteal muscle contracture
赵晨光 1贺西京 1陆斌 1李浩鹏1
作者信息
- 1. 西安交通大学医学院第二附属医院骨二科,陕西西安710004
- 折叠
摘要
目的:探索凋亡相关基因Bc1-2和Bax在臀肌挛缩带中的表达情况及其对臀肌挛缩症(GMC)发病的作用.方法:收集臀肌挛缩症患者挛缩带和病变旁肌肉的病理标本,运用逆转录-聚合酶链式反应(RT-PCR)、免疫组化和West-ern blot技术检测Bc1-2和Bax在mRNA水平和蛋白水平的表达情况.结果:Be1-2定位于成纤维细胞的胞质中,呈强阳性表达,其在mRNA水平和蛋白水平分别上调了6.1倍和3.9倍(P<0.05).Bax也定位于成纤维细胞的胞质中,呈弱阳性表达,其在mRNA水平和蛋白水平的表达未见显著性上升(P>0.05).结论:抗凋亡基因Be1-2与凋亡基因Bax的对抗作用失调可能是引起挛缩带产生的重要机制.
Abstract
AIM: To explore expression and effect of apoptosis-related gene Bcl-2 and Bax on gluteal muscle contracture( CMC). METHODS: Harvest contraction band and adjacent muscle from GMC patients and explore the expression of Bcl-2 and Bax using immunohistochemistry, Reverse transcription and polymerase chain reaction ( RT-PCR ) and Western bolt analysis. RESULTS:Bcl-2 was intensely expressed in fibroblast cells. Its expression increased 6. 1-fold and 3. 9-fold at mRNA level and protein level, respectively ( P < 0.05). Bax was faintly expressed in fibroblast cells. However, the expression of Bax was not increased at both of the levels ( P > 0. 05 ). CONCLUSION: Unbalanced change of Bcl-2 and Bax may initiate and propagate the fibrotic cascade in the gluteal muscles of GMC patients.
关键词
臀肌挛缩症/纤维化/Be1-2/BaxKey words
gluteal muscle contracture/fibrosis/Bax/Bcl-2引用本文复制引用
出版年
2009
NETL
NSTL
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