HYAL2基因沉默对卵巢癌SKOV3细胞增殖和侵袭的影响
RNAi-mediated knockdown of HYAL2 gene inhibits proliferation and invasion in human ovary carcinoma SKOV3 cells
周敏 1周琦 2李少林 3唐良萏 1赵迎泽4
作者信息
- 1. 重庆医科大学附属第一医院妇产科,重庆400016
- 2. 重庆市肿瘤研究所,重庆400016
- 3. 重庆医科大学放射医学教研室,重庆400016
- 4. 重庆医科大学医学检验系,临床检验诊断学省部共建教育部重点实验室,重庆400016
- 折叠
摘要
目的:利用RNA干扰(RNAi)技术抑制人卵巢癌SKOV3细胞株HYAL2基因的表达,探讨siRNA-HYAL2对卵巢癌SKOV3细胞增殖和侵袭能力的影响.方法:将靶向HYAL2的siRNA及对照分别转染人卵巢癌SKOV3细胞株,RT-PCR测定HYAL2基因mRNA表达情况,MTT法检测SKOV3细胞增殖,FCM检测细胞周期变化,构建Transwell小室体外侵袭模型观察其对细胞侵袭能力的影响.结果:转染siRNA-HYAL248 h后HYAL2基因mRNA表达水平明显降低,细胞存活率下降(P<0.05),s期细胞百分比降低、G0/G1期细胞百分比升高(P<0.05);转染24 h后平均穿膜细胞数减少(P<0.05).结论:siRNA-HYAL2能特异性抑制HYAL2的mRNA表达水平,HYAL2基因沉默后,卵巢癌SKOV3细胞增殖和侵袭能力降低,HYAL基因可能成为卵巢癌基因治疗的新靶点.
Abstract
AIM: To study the effect of RNA interference against HYAL gene on the proliferation and invasion in ovarian epthelial carcinoma SKOV3 cell line. METHODS: The transcription of HYAL gene was detected by RT-PCR; the proliferation was analysed by MTT-assay and flow cytometric; penetrating matrigel capacity of the SKOV3 cells was detected by the pattern of vitro invasion. RESULTS: After RNA interference against HYAL gene for 48 h, HYAI2 mRNA was downregulated; the proliferation of the SKOV3 cell was inhibited obviously; flow cytometry showed that cells in S phase were reduced ( P < 0. 05), with more cells arresting in G0/G1 phase of cell cycle ( P < 0. Penetrating matrigel capacity inhibited significantly (P < 0. 05). CONCLUSION: The capcity of proliferation and invasion can be inhibited by RNA interference against HYAL gene. It indicates that HYAI2 gene is likely to become new potential target of gene therapy approach to ovarian epithelial carcinoma.
关键词
卵巢肿瘤/RNA干扰/透明质酸酶/增殖/侵袭Key words
ovarian neo plasm/RNA interference/hyaluroni-dase/proliferation/invasion引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据