Establishment and evaluation of rabbit osteoblast cell culture model based on BMSCs
AIM: To establish a cell culture model for bone disease research, investigate rabbit BMSCs' growth and differentiation in vitro. METHODS: Bone marrow mesenehymal stem cells were cultured by density gradient centrifugation and attachment, random select two group from the third generation. Group A; induced by low carbohydrates DMEM; Group B; negative group. The growth of rBMSCs was observed inverted-microscopically, CD44,CD90 were detected, also the HE and ALP stains, assess the activity of cell and counter the mineralized nodus. RESULTS: There were no significant difference between each groups after 7 d culture, the cells apparent long fusiform shape, ALP stains shows significant positive in group A compared with group B after 2 weeks. Mineralized nodus counters show significant higher in group A compared with group B in alizarin red stain after 21 d culture ( P < 0. 05). CON CLUSION; Co-application of density gradient centrifugation and attachment in BMSCs cuture shows high capacity of ossification and better activity of ALP, which can provide a cell culture model for bone tissue engineering.
NETL
NSTL
万方数据
维普
