摘要
目的:了解40岁以下发病的糖尿病患者的临床特点以及胰淀素(IAPP)Ser20Gly突变在该人群的发生情况.方法:常规方法测定234例40岁以下发病糖尿病患者的糖脂代谢、GAD抗体、胰岛素抵抗,分析其家族史和治疗情况并用PCR-RFLP和直接序列分析方法对IAPPSer20GIy突变情况进行筛查.结果:糖尿病患者的FBG,PBG2h明显高于正常人(P<0.001),TG增高(P<0.05),平均HbAlc(10.4±2.4)%,HOMAIR 3.63～8.60.GAD-Ab阳性者占3.42%.有糖尿病家族史者占61.54%.饮食、运动疗法和口服药物治疗者分别占40.6%,41.03%,而胰岛素治疗者有18.65%.在234例糖尿病患者中发现2例IAPP基因Ser20Gly突变者,其中1例为纯合子,另1例为杂合子突变.结论:40岁以下发病糖尿病患者存在明显代谢异常和胰岛素抵抗.IAPP Ser20Gly突变在该人群发生率约为0.85%.显高于正常人(P<0.001),TG增高(P<0.05),平均HbAlc(10.4±2.4)%,HOMAIR 3.63~8.60.GAD-Ab阳性者占3.42%.有糖尿病家族史占61.54%.饮食、运动疗法和口服药物治疗者分别占40.6%,41.03%,而胰岛素治疗者有18.65%.在234例糖尿病患者中发现2例IAPP基因Ser20Gly突变者,其中1例为纯合子,另1例为杂合子突变.结论:40岁以下发病糖尿病患者存在明显代谢异常和胰岛素抵抗.I
Abstract
AIM: To study the clinical characters and the incidence of the amylin gene Ser20Gly mutation. METHODS: In 234 diabetic patients who onset diabetes before the age of 40 the parameters of the glucose and fat metabolism, anti-GAD antibody, the rate of diabetic family positive and insulin resistance were measured using routine methods. By PCR-RFLP plus direct sequencing to detect the mutation of the amylin gene Ser20Gly mutation. RESULTS: The FBG, PBG2h and TG of early onset diabetic patients were higher than the normal health group's. HbAlc was (10.4 ± 2. 4)%. HOMAIR was 3. 63-8. 60. The rate of GAD autoantibody positive was 3. 64%. There were more than 80% diabetic patients used diet and oral agent therapy. 18% of those patients used insulin treatment. Two patients have Ser20Gly mutation of amylin gene. One is a homozygous another one is heterozygous. CONCLUSION: There are severe insulin resistance and poor glucose control in the early onset diabetic patients. The prevalence of the amylin gene Ser20Gly mutation is 0. 85% in those early onset diabetic patients.
NETL
NSTL
维普
万方数据