Reverse transcription in situ PCR detection of borna disease virus infection
AIM: To establish reverse transcription in situ PCR (RT ISPCR) detection of borna disease virus (BDV) infection and compare it with RT-PCR and ELISA detection. METHODS: The OL cells which used as BDV detection model were transfected by plasmid, BDV-GFP-P24, and tested by RT-PCR. All the BDV positive samples and negative controls ( both human and animal) were tested by RT ISPCR, RT-PCR and ELISA. RESULTS:The BDV detection model was confirmed by RT-PCR. 4 samples were detected positive and 10 samples were negative by RT IS PCR. The results were only slight different to that of RT-PCT ( 1 suspected positive when RT ISPCR is positive) and ELISA ( 1 suspected positive when RT ISPCR is negative). CONCLUSION :RT ISPCR can be used to detection of human and animal infection of BDV and it can be used in the study of BDV infection mechanism.
borna disease virusreverse transcription in situ PCRreal-time PCRinfection
NETL
NSTL
万方数据
维普
