Rapid diagnoses of avian influenza virus and avian influenza H5 virus by real-time PCRs
AIM: To detect avian influenza virus subtype H5 by the real-time fluorescent quantitative PCRs were established. METHODS: It were designed that the primers and probes in universal assay based on AIV M gene and those in subtype H5 assay based on AIV H5 gene, and was optimized in a reaction system and a condition to improve the sensitivity, specificity and reproducibility. RESULTS:After optimization in the reaction system and the condition, the sensitivities of the universal assay and the H5 assay reached to 1 × 10~(1.25) EID_(50); the Kappa coefficient between the cell culture and the Real time PCR of AIV H5 is 0.991 (P <0. 01) , which statistically showed that there was the strong coincidence between two METHODS: the CV values of CT in the intra and inter batches were far less than 10% , which statistically showed that there was the excellent reproducibility. CONCLUSION:There were the excellent qualities of both assays in this studies in the sensitivity, specificity and reproducibility, which might been suggested for use to detect in avian influenza epidemic or human avian influenza H5 case.
NETL
NSTL
万方数据
维普
