Titanium coat for hepatic lobe in the preparation of a hepatic cirrhotic and portal hypertension animal model in dogs
AIM: Titanium reticular layers was connected as a coat for hepatic lobes in order to rapidly make a hepatic cirrhosis dog model, which would be much similar to real cirrhosis and portal hypertension for further hepatic research work. METHODS: Twenty healthy cross-breed dogs were selected, and their hepatic function, PVP and PVF, size of the spleen and hepatic tissue were all collected preoperatively as control. The left two lobes of all the dogs were removed, and titanium reticular layers were connected as coats according to the size of the other three lobes. The hepatic function, PVP and PVF, size of the spleen and hepatic tissue were collected again 4 weeks later. RESULTS: Two lobes removal and titanium reticular coats wearing for the other three lobes resulted in reduced hepatic tissue, obviously distorted blood vessels, intrahepatic inflamation and collagen increasing, which distinctly simulate the real blood flow and pathologic changes of hepatic cirrhosis. The hepatic function decreased obviously at the end of the 4th week after the operation, whereas the PVP increased obviously. The varicosities of the branches emerged while the spleen enlarged extraordinarily and the end of the 4th week. CONCLUSION: Titanium coats wearing of hepatic lobes proved to be a good method for the preparation of dog hepatic cirrhosis model. The whole course of the experiment lasted only 4 weeks. The removal of the two lobes resulted in reduced hepatic cells, while the titanium coats limited the compensation of the other three lobes. Titanium needles within the lobes induced the chronic inflammation and increased collagen, which then finally resulted in distorted blood flow within, varicosities, enlarged spleen and decreased hepatic function.
NETL
NSTL
万方数据
维普
