Effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside against MPP~+ induced apoptosis in PC12 cells
AIM:To study the protective effect of 2,3,5,4,-tetrahydroxystilbene-2-O-β-D-glucoside(TSG)on the injury of PC12 cells induced by 1-methy-4-phenylpyridinium(MPP~+).METHODS:Using MPP~+ induced PC12 cells damage as the model of Parkinson's disease in vitro,the protevtive effect of TSG was explored.The cytotoxicity of MPP+~ was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyhetrazolium bromide(MTT)assay,the morphological change of the nuclear was detected by Hoechest 33258 staining,the apoptotic percentage of PC12 cells was detected by flow cytometry(FCM)and the fragmentation of DNA was assessed with TUNEL staining.RESULTS:1,5,10 μmol/L TSG respectively played a protective role against MPP~+ induced PC12 cells injury.Compared with MPP~+ treated cells,after exposure with 1,5 and 10μmol/L TSG,cell viability was increased to (57.8 ±1.2)%,(74.3 ±2.7)%,(86.8±2.0)%,respectively. Hoechest 33258 staining demonstrated that more cells were characterized by nuclear condensation in MPP~+ group,while in the group of TSG,the rate was decreased to(31.6 ±2.3)%.(22.4 ±1.8)%and(13.4 ±1.1)%,respectively.FCM analysis indicated that apoptotic ratio was decreased to 27.2%.19.1%and 13.3% after TSG(1,5 and 10 μmol/L)pretreatment,while in control and MPP~+ group,the ratio wag 1.5%and 35.6%.TUNEL staining indicated that TUNEL-positive cells were increased in the MPP~+ treated group and markedly reduced to(25.3 ±1.2)%,(17.3±0.9)%and(11.7 ±0.9)%respectively after pretreated with TSG.CONCLUSION:The data indacates that TSG has a protective effect on the injury of PC12 cells induced by MPP~+.
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