钩吻素子对神经胶质瘤细胞U251生长抑制及诱导凋亡作用
Growth-inhibiting and apoptosis-inducing effects of Kou on human glioma cell line U251
王万山 1薛侠 2王达安 3迟德彪4
作者信息
- 1. 南方医科大学比较医学研究所,广东,广州,510515
- 2. 陕西省妇幼保健院,陕西,西安710001
- 3. 暨南大学医学院,广东,广州,510630
- 4. 南方医科大学基础医学院,广东,广州,510515
- 折叠
摘要
目的:探讨钩吻素子体外对神经胶质瘤细胞U251的生长抑制作用及诱导凋亡的作用.方法:利用MTT法和台盼蓝拒染法观察钩吻素子对神经胶质瘤细胞U251生长抑制作用.应用激光共聚焦、流式细胞仪、电子显微镜检测钩吻素子诱导U251细胞的凋亡作用.结果:MTT结果显示钩吻素子在(5～50 mg/L)的浓度范围内对人神经胶质瘤U251细胞具有显著的抑制作用.生长曲线结果显示药物的各个浓度对U251的抑制作用旱时间依赖性.流式细胞仪可以观察到明显的"亚GI期"峰(凋亡峰),且凋亡率达到22.4%.激光共聚焦显微镜可以观察到典型的凋亡核形,如核同缩和核碎裂等.透射电子显微镜下见到典型的早期凋亡形态学表现.结论:在一定的浓度范围内,钩吻素子可以抑制U251细胞的生长,并呈剂量和时间依赖效应.其抗肿瘤的特性与引起肿瘤细胞的凋亡有关.
Abstract
AIM:To investigate the growth-inhibiting and apoptosis-inducing effect of Kou on human glioma cell line U251 in vitro.METHODS:MTT assay and trypan blue exclusion method were applied to observe the growth-inhibiting effect of Kou on human glioma U251 cells.The apoptosis-inducing effects of Kou on U251 were examined with flow cytometry(FCM),laser scanning confocal microscope(LSCM)and electron microscope(EM).RESULTS:MTY assay showed that Kou had remarkable inhibitory effect on U251 in the concentration ranges of 5-50 mg/L,and cell curve measurement results indicated the inhibitory effect displayed in a time-dependent manner.FCM assay showed a evident apoptosis peak and the apoptotic rate Was 22.4%.Marked morphoiogical changes such as karyopycnosis and nuclear fragmentation of cell apoptosis were observed very clearly by Hoechst 33342 fluorescence staining.Typical early apoptotic appearance was also seen under EM.CONCLUSION:Kou exhibits dose-and timedependent growth-inhibiting effects on U251 cells in a certain concentration range,its antineoplastic characteristics are related with tumor its apoptosis-inducing effect on cells.
关键词
钩吻素予/凋亡/神经胶质瘤细胞Key words
Kou/apoptosis/human glioma cell lines引用本文复制引用
基金项目
广东省重点科技攻关项目(2002A30103010)
出版年
2009
NETL
NSTL
维普
万方数据