组织工程皮肤与正常皮肤组织学差异的实验研究
Experimental research of histological difference between tissue-engineered skin and normal human skin
李媛 1陆伟 2冀堃 3刘源 1董绍忠 1金岩2
作者信息
- 1. 第四军医大学口腔医学院口腔组织病理学教研室,陕西,西安,710033
- 2. 第四军医大学口腔医学院口腔组织病理学教研室,陕西,西安,710033;第四军医大学组织工程研发中心,陕西,西安,710033
- 3. 第四军医大学组织工程研发中心,陕西,西安,710033
- 折叠
摘要
目的:研究组织工程皮肤与正常皮肤表皮层组织学的差异,并探讨其意义.方法:用组织工程方法构建人全层皮肤,以正常在体皮肤作为对照,组织学、免疫细胞化学、透射电镜观察表皮层的异同.结果:组织工程皮肤与正常皮肤在组织学上有一定差异,缺少上皮钉突,CK19在组织工程皮肤成熟期表达阴性,但CK10和pan-CK在两者表达皆为阳性,透射电镜观察上皮结构接近正常皮肤.结论:组织工程皮肤表皮层与真皮层结合不如正常皮肤紧密,可能与缺少上皮钉突有关,其表皮细胞基本分化成熟.
Abstract
AIM:To study the differences in histology of the tissue-engineered skin and normal human skin.METHODS:The bilayer skin replacement waft constructed by tissue engineering method,compared with the normal human skin by histological and immunohistochemistry methods.RESULTS:There were some differences in tissue-engineered skin and the normal skin.Epidermis rete pegs were presented only in the normal skin,CK19 was negative in the matured tissue engineered-skin,but both CK10 and pan-CK were positively expressed in tissue-engineered skin and the normal skin.Epithelium structure by TEM wag similar to the normal skin.CONCLUSION:The dermo-epidermal junction in tissue-engineered skin was less tighter than normal skin,may be due to the absence of epidermis rete pegs,epidermal cells had difierentiated.
关键词
组织工程皮肤/正常皮肤/再生Key words
tissue-engineered skin/normal skin/regeneration引用本文复制引用
基金项目
国家高技术研究发展计划(863计划)组织工程皮肤重大专项基金(2006AA02A119)
陕西省社发攻关项目(2009K14-03)
出版年
2009
NETL
NSTL
维普
万方数据