病理性瘢痕与正常皮肤的蛋白质组学研究
Analysis of differential expressed proteins between pathological scars and normal skin
李晖 1刘广忠 1孙伟民 1薛宏斌1
作者信息
- 1. 延安大学附属医院烧伤科,陕西,延安,716000
- 折叠
摘要
目的:利用蛋白质组学的研究方法,找出增牛性瘢痕、瘢痕疙瘩与正常皮肤组织成纤维细胞的差异蛋白质,为防治病理性瘢痕提供新思路.方法:应用2-DE技术建立三种组织细胞的蛋白质图谱,分析后找到差异蛋白点,用质谱分析仪及数据库确定蛋白质的种类.结果:选择增生性瘢痕、瘢痕疙瘩和正常皮肤表达的差异蛋白9个,鉴定出差异蛋白5个,分别为:磷脂酰胆碱结合蛋白、银屑病型脂肪酸结合蛋白、Ⅰ型胶原蛋白、Ⅲ型前胶原蛋白、Ⅰ型前胶原蛋白.结论:共找出增牛件瘢痕、瘢痕疙瘩与正常皮肤间5种差异蛋白,为研究病理性瘢痕的发病机制提供了一种新方法,也为临床用药研究提供了新方向.
Abstract
AIM:To use proteomics method find differential expressed proteins of two-dimensional gel electrophoresis(2-DE)profiles of human hypertrophic scar,keloid and normal skin tissue,and to provide a new method for therapy of human pathological scars.METHODS:To establish and analyze two-dimensional gel electrophoresis(2-DE)profiles of hypertrophic seals,and normal skin.Identified part of differential expression proteins and identifled by MALDI-TOF-MS.RESULTS:2-DE profiles of human hypertrophic scar,keloid and normal skin tissue were compared 9 differential expression protein sports were selected and 5 differential expression protein were identified;the proteins were:phosphatidylethanolamine-binding protein,fatty acid binding protein,type Ⅰ collagen.carboxy-propeptide of alpha Ⅲ procollagen,carboxy-propeptide of alpha Ⅰ procollagen.CONCLUSION:In this study,5 differential proteins among human hypertrophic scar,keloid and normal skin tissue were identified.It may provide a new therapy and method system for study of the pathogenesis mechanism of pathological scars.
关键词
病理性瘢痕/增生性瘢痕/瘢痕疙瘩/蛋白质组学/差异蛋白Key words
pathological scars/hypertrophic scar/keloid/proteomics/differential expression protein引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据