Mechanisms of group Ⅲ mGluRs against cultured neuron apoptosis induced by β-amyloid peptide 31-35
AIM:To determine the mechanisms of neuroprotection by group Ⅲ metabotropic glutamate receptors(mGluRs)activation against apoptosis induced by 13-amyloid peptide 31-35 (Aβ31-35)in frontal lobe cortical neurons.METHODS:The cerebral cortical neurons were incubated with A1331-35(25 μmol/L)or coapplied with group Ⅲ mGluRs agonist of L-SOP or(R,S)PPG(100μmol/L)for 24 h.The apoptosis of the cells was analyzed by flow cytometry and easpases activity.RESULTS:L-SOP or(R,S)-PPG(100 μmol/L)significantly inhibited the neuron apoptosis and decreased caepase-3,-8 and-9 in the Aμ31-35 treated neurons.CONCLUSION:The results described in the present study suggested that activation of group Ⅲ mGluRs protected neurons from Aβ31-35-induced apoptosis by blocking the caspase-dependent intrinsic and extrinsic apoptotic pathways.
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