Berberine inhibits lipopolysaccharide induced COX-2 expression via ERK signaling cascade pathways in human peripheral blood monocytes
AIM:To investigate the inhibition of berberine (BBR)on lipopolysaccharide(LPS)induced COX-2 expression via ERK signaling cascade pathways in human peripheral blood monocytes(HPMC).METHODS:HPMC were isolated and cultured from whole blood and divided into 5 groups treated with null,LPS,LPS+BBR 25 μmol/L,LPS+BBR 50 μmol/L,LPS+BBR 100 μmol/L respectively for 30 min,6,12 and 24 h.Then monocytes were extracted for RT-PCR and westemblot analysis to examine COX-2 mRNA and protein activated expression of ERK signaling pathway.Meanwhile,ERK and inhibitor was incubated together with LPS stimulation to examine COX-2 mRNA and protein expression.RESULTS:At the four time points after treatment,the COX-2 mRNA and protein expression decreased at a low ebb at 12 h after BBR treatment(P<0.05).In the group of BBR treatment at various time points(at 6,12,24 h after treatment)and three levels of dosage,ERK protein expression was inhibited significantly.At the concentration of 25 and 50 μmol/L,JNK protein expression was not inhibited significantly by BBR compared with LPS stimulation group(P>0.05).Whereas with the treatment of BBR at the concentration of 1 00 μmol/L,JNK protein expression could be inhibited obviously compared with LPS stimulation group(P<0.01).Human monocytes COX-2 mRNA and protein expression was inhibited statistically significantly while incubated with ERK and JNK pathway inhibitor,PD98059(P<0.01).CONCLUSION:We conclude that BBR inhibits COX-2 mRNA and protein expression in a dose-dependent manner and ERK expression could be suppressed by BBR.BBR may inhibit COX-2 expression via ERK signaling pathway in HPMC.High concentration of BBR may inhibit COX-2 expression via JNK signaling pathway in HPMC.
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