Effects of local wet hyperthermia combined with microwave ablation on the proportion and function of dendritic cells in animal model of mouse roammary carcinoma
AIM:To investigate the effects of local wet hyperthermia combined with microwave ablation on the proportion and function of dendritic cells(DCs)in animal model of mouse mammary carcinoma and to explore its significance.METHODS:An experimental animal model of mouse mammary carcinoma was established and then treated with local wet hyperthermia combined with microwave ablation.The proportion of DCs in splenocytes from tumor bearing mice was firstly detected by FACS.The expression of MHC class Ⅱ molecule,as well as co-stimulatory molecule CD80 and CD86,on DCs were also analyzed by FACS.The proliferation of T cells stimulated by DCs was determined by MTT assay.Moreover,the IFN-γ and IL-4 production of T cells induced by DCs were also analyzed by FACS.RESULTS:The proportion of DCs and the expression level of MHC class Ⅱ molecule and CD86 on DCs in local wet hyperthermia combined with microwave treated group were significantly higher than those of microwave ablation or local wet hyperthermia treated alone group(P<0.05).Furthermore,compared with control groups,botll the proliferation and IFN-γ production of T cells stimulated by DCs increased significantly in local wet hyperthermia combined with microwave treated group.CONCLUSION:Local wet hyperthermiacombined with microwave ablation more effectively increases the proportion and function of DCs than single local wet hyperthermia or microwave ablation.
local wet hyperthermiamicrowave ablationdendritic cellscytokine
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NSTL
万方数据
维普
