Effect of NADPH oxidase-4 on hypertension induced myocardial hypertrophy in rat
AIM:To investigate the mRNA expression of NADPH oxidase-4(NOX-4)and its effect on hypertension induced cardiac hypertrophy in rat.METHODS:Model of over-loading myocardial hypertrophy wag established through coarctation of abdominal aorta.Fourty-eight rats were randomly divided into 4 groups:Control group,model group,4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl(Temp01)-treatment group and Diphenylene iodoniam(DPI)-treatment group.Tempol and DPI were administrated in the drinking water after the operation for the rats of the two treatment groups.Systolic blood pressure(SBP)was measured before the operation as well as 2,4 and 8 weeks after the operation.Further,at 8 weeks after the operation,heart weight indexes(LVW/BW and HW/BW)were measured to determined the level of cardiae hypertrophy.Hydrogen peroxide(H_2O_2)level was measured and the NOX4 mRNA was detected for all rats at 8 weeks after the operation.RESULTS:SBP of the model group increased 2 weeks after the operation and was higher than SBP of the control group at all time points after the operation(P<0.01).SBP's of both Tempol-and DPI-treatment groups were the same as that of the model group at 2 weeks after the operation,but became lower than SBP of the model group at 4 and 8 weeks after the operation(P<0.05).For the model group,the LVW/BW,HW/BW,H_2O_2 and NOX4 mRNA levels are higher than those of the control group at all time points after the operation(P<0.01).For the DPI-treatment group,the above parameters were all lower than those of the model group(P<0.05).For the Tempol-treatment group.the LVW/BW,HW/BW and H,O,level were markedly lower than those of the model group(P<0.05),whereas the NOX4 mRNA level was the same as that of the model group.CONCLUSION:Hypertension induced cardiac hypertrophy is partly medicated by NOX4 and its downstream ROS.
NETL
NSTL
万方数据
维普
