Dynamic changes of kCNE1,KCNE2 gene expression in ischemic myocardium of rat
AIM:To investigate the dynamic changes of KCNE1,KCNE2 gene expression in acute ischemic myocardium of rat,and to explore the mechanism of cardiac arrhythmia after acute myocardial infarction(AMI).METHODS:Acute myocardial infarction model of Sprague-Dawley rat was successfully established.KCNE1,KCNE2 mRNA and protein levels were detected in ischemic myocardium using reverse transcriptase PCR and Western blotting 24 h,1,2 and 4 weeks after the establishment of AMI model,and ventricular arrhythmia was detected using ECG in all groups.RESULTS:The expression level of both KCNE1 and KCNE2 increased significantly in ischemic myocardium(P<0.05)24 h after AMI,achieving highest level within 1-2 weeks.and then decreased.Nevertheless.the mRNA and protein level of KCNE1 and KCNE2 remained significantly higher in ischemic myocardium 4 weeks after AMI,compared with that in myocardium of control group(P<0.05).The incidence of ventricular arrhythmia was significantly higher in AMI groups than in sham operation group,especially during the period from 1 week to 2 week after the establishment of AMI model.CONCLUSION:The incrcased expression level of KCNE1 and KCNE2 gene in ischemic myocardium after acute myocardial infraction may have influence on the electrophysiological characteristics of these two ionophorous protein,resulting in the alteration of auto-rhythmicity,excitability and conductivity of myocardial cells,and therefore inducing cardiac arrhythmia after acute myocardial infarction.
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万方数据
维普
