乌拉坦增强延长离体大鼠心脏的低温保存效果
Myocardial protective effects of urethane on isolated rat heart in prolonged hypothermic preservation
吴铁军 1毕生辉 1崔勤 1金振晓 1易定华 1刘维永1
作者信息
- 1. 第四军医大学西京医院心血管外科,陕西,西安,710033
- 折叠
摘要
目的:观察乌拉坦埘延长离体大鼠心脏的低温保存作用.方法:成年雄性SD大鼠分为两组(n=6):对照组:HTK保存液;试验组:HTK保存液加乌拉坦(30 mmol/L).心脏分别置入两种保存液4℃下保存6和18 h.采用Langendorff 心脏灌注装置进行保存前及保存后再灌注30 min时心脏功能测定,比较心脏功能恢复率、ATP含量、细胞凋亡和超微结构变化.结果:低温保存后实验组左窒功能恢复率较对照组明显升高,细胞凋亡及超微结构损伤较对照组减轻;保存18 h后实验组ATP含量显著高于对照组.结论:乌拉坦加入HTK心脏保存液能够显著增强离体大鼠心脏的低温保存效果.
Abstract
AIM:To observe the protective effects of urethane plus HTK on the isolated rat hearts in prolonged hypothermic preservation.METHODS:Male Sprague-Dawley rats(250-280 g)were divided into 2 groups(n=6 per group)according to each preservation solution used:control group,HTK solution;experimental group,HTK solution plus urethane.The hearts were arrested and stored in each preservation solutions respectively for 6 and 18 h at 4℃.After preservation,the myocardial function,ATP content,cardiomyocyte apoptosis and uhrastrueture were examined.RESULTS:Compared with control group,experimental group showed higher reeovery of cardiac.function,lower rate of cardiomyocyte apoptosis and better cardiomyocyte uhrastructure both for 6 and 18 h of preservation.The ATP content after 18 h of preservation in control group was significantly higher than that in experimental group.CONCLUSION:Urethane led to significandy better and longer preservation in isolated hearts subjected to prolonged hypothermic preservation.
关键词
乌拉坦/器官保存/心脏移植Key words
urethane/organ preservation/heart transplantation引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据