β-arrestin 2 inhibits TNFα induced IKKα/β activation by interacting with RIP1
AIM:To investigate the effects of β-arrestin 2 on TNFα signal transduction.METHODS:β-arrestin 2 was overexpressed or knockdowned in HT-29 cells,and HT-29 cells were stimulated with different time period,signal transduction ofIKKα/β.IkBα TAKl JNK p38 and the potential mechnism were detected by Western Blot and protein interaction Was detected by immuno-precipitation.RESULTS:The research reports here for first time that β-arrestin 2 inhibits TNFα induced IKKα/β and its downstream signaling activation by interacting with RIP1 and inhibiting its polyubiquitination,promotes TAK1 and downstream signaling activation.Interfering the expression of β-arrestin 2 reverse above resuIts.CONCLUSION:β-arrestin 2 inhibits TNFαinduced IKKα/β activation and promotes TAK1 activation by interacting with RIP1 and inhibiting its polyubiquitination.
NETL
NSTL
万方数据
维普
