C-FLIP基因在甲基硫代腺苷诱导结肠癌RKO细胞凋亡过程中的作用
Effects of C-FLIP on the process of methylthioadenosine induced apoptosis in human colon cancer RKO cells
张青松 1李东坤 1朱理玮2
作者信息
- 1. 华北煤炭医学院附属开滦医院普外科,河北唐山063000
- 2. 天津医科大学总医院普外科,天津300052
- 折叠
摘要
目的:探讨甲基硫代腺苷(MTA)对结肠癌RKO细胞凋亡的作用及其相关机制的研究.方法:采用Hoechst33258染色技术检测MTA对结肠癌RKO细胞凋亡的影响,并采用Readtime PCR和Western Blot的方法在mRNA和蛋白水平研究cFLIP基因在MTA诱导的结肠癌RKO细胞凋亡过程中的作用.结果:MTA对结肠癌RKO细胞具有明显促进凋亡的作用.在这一过程中,cFLIP基因在mRNA和蛋白水平上均出现明显卜调情况.结论:MTA对RKO细胞具有明显凋亡诱导作用,cFLIP基因的下调町能起到了重要的作用.
Abstract
AIM:To study the effects and its mechnism of methvlthioadenosine on apoptosis in RKO cells.METHODS:Hoechst 33258 staining was used to detect the effects of methyhhioadenosine on apoptosis in RKO cells,and the mRNA and protein level of cFLIP in this course was detected with the mothed of Realtime PCR and Western Blot.RESULTS:Methylthioadenosine could significantly induce apoptosis of RKO,and mRNA and protein level of cFLIP were downregulated significantly in this course.CONCLUSION:Methyhhioadenosine could induce apoptosis in RKO cells,and the downregulation of cFLIP gene misht olay a major role.
关键词
甲基硫代腺苷/细胞凋亡/cFLIP/结肠肿瘤/RKO细胞Key words
methyhhioadenosine/apoptosis/cFLIP/colon cancer引用本文复制引用
基金项目
唐山市科技局科学技术研究及发展计划项目(07130229D)
出版年
2009
NETL
NSTL
维普
万方数据