Effect of culture supernatant of Toxoplasma gondii on human ovarian cancer cell line SKOV-3
AIM:To investigate the inhibitive effects of cuhure supernatants of Toxoplasma gondii on the proliferation of human ovarian cancer cell line SKOV-3 and/or its ieduced apoptosis in vitro.METHODS:SKOV-3 cells(concentration of 5×10~7/L)were harvested in the different cell culture plates.The cells were treated for different hours with different concentrations of Toxoplasma gondii culture supernatant(the concentration of tachyzoites was 3×10~(10)/L,6 ×10~(10)/L,12×10~(10)/L respectively).Growth inhibition rates were measured with the MTY method.Apoptosis of the SKOV-3 cells was detected with the following.FCM was used to test the rates of apoptosis of the cancer cells stained with the fluorescent dye of Annexin-v-FITC/PI,fluorescence microscopy was used to observe the changes of eeUular morphology,the agarose electrophoresis was used to detect the DNA changes of SKOV3 cells.RESULTS:The culture supernatant of Toxoplasma gondii inhibited the proliferation of SKOV-3 cells and The growth inhibition rates were increasing with the time and the concentrations,The higheat rate can reach(33.52±2.63)%at the 48 th hour when the concentration was 12×10~(10)/L.The classic cellular morphology of apoptosis in the treated SKOV-3 ceHs were observed under the fluorescence microscope.DNA fragments in the treated cells were revealed by the agarose electrophoresis.In the flow cytometric analysis,a maximum apoptosis rate of 18.23% was detected at 48 h of treatment with the supernatants,but the apoptosis rates decreased and dead rate increased at the treatment time beyond 48 h.CONCLUSION:The culture supematant of Toxoplasma gondii reveals an inhibition to the proliferation of SKOV-3 cells in vitro,and induces their apoptosis in vitro.
Toxoplasma gondiihuman ovarian cancer cell line SKOV-3apoptosis
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NSTL
万方数据
维普
